Literature DB >> 2123414

Purification of swine haptoglobin by affinity chromatography.

T E Eurell1, W F Hall, D P Bane.   

Abstract

A globin-agarose affinity chromatography technique was used to purify swine haptoglobin. This technique provides a highly specific, single-step purification method without the contamination of extraneous serum proteins reported by previous studies. Complex formation between the haptoglobin isolate and swine hemoglobin confirmed that biological activity was maintained during the purification process. Immunoelectrophoretic and Ouchterlony immunodiffusion methods revealed that the swine haptoglobin isolate cross-reacted with polyvalent antisera against human haptoglobin.

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Year:  1990        PMID： 2123414      PMCID： PMC1255700     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  5 in total

1.  The purification of porcine haptoglobin by affinity chromatography with concanavalin A-Sepharose.

Authors:  F A Terpstra; D B Smith
Journal:  Can J Biochem       Date:  1976-11

2.  Studies on swine and canine serum haptoglobins.

Authors:  B S Shim; C S Yoon; S K Oh; T H Lee; Y S Kang
Journal:  Biochim Biophys Acta       Date:  1971-07-25

3.  The hemoglobin-haptoglobin bond. I. Dissociation of the complex and recovery of the native haptoglobin in an affinity chromatography system.

Authors:  J Javid; J C Liang
Journal:  J Lab Clin Med       Date:  1973-12

4.  Distinction between heme and hemoglobin-binding proteins of swine and rabbits by electrophoresis.

Authors:  A A Grunder
Journal:  Vox Sang       Date:  1968       Impact factor: 2.144

5.  Studies on porcine haptoglobin and its complex with human hemoglobin.

Authors:  I H Fraser; D B Smith
Journal:  Can J Biochem       Date:  1971-02
  5 in total
  2 in total

1.  Serum haptoglobin concentration as an indicator of weight gain in pigs.

Authors:  T E Eurell; D P Bane; W F Hall; D J Schaeffer
Journal:  Can J Vet Res       Date:  1992-01       Impact factor: 1.310

2.  Serum haptoglobin concentration in growing swine after intranasal challenge with Bordetella bronchiseptica and toxigenic Pasteurella multocida type D.

Authors:  C J Francisco; T R Shryock; D P Bane; L Unverzagt
Journal:  Can J Vet Res       Date:  1996-07       Impact factor: 1.310
  2 in total

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