BACKGROUND: Vacuum-assisted closure induces microdeformations of the wound surface and accelerates healing of complex wounds; however, a thorough understanding of the biology of cellular mechanotransduction is lacking. We hypothesized that fibroblast shape and function can be altered in an in vitro vacuum-assisted closure device. METHODS: A 3-dimensional fibrin matrix with cultured murine fibroblasts and an intervening polyurethane foam was exposed to 125 mm Hg suction and compared with similar wells without suction. We measured fibroblast proliferation and morphology using fluorescence microscopy and gene expression change using real-time reverse-transcriptase polymerase chain reaction at 24, 48, and 72 hours. RESULTS: Wells exposed to suction induced significant proliferation of fibroblasts and morphologic changes visible by larger, rounder, and notable dendrite-like branching and process extensions. Type 1 collagen alpha 1 (COL1A1), fibroblast growth factor 2 (FGF2, bFGF), and transforming growth factor beta 1 (TGFβ1) were all up-regulated after 48 hours of exposure to suction. Smooth muscle actin alpha 2 (Acta2, α-SMA) was up-regulated after 72 hours. CONCLUSIONS: Microdeformations produced by the combination of polyurethane foam and suction are associated with increased fibroblast proliferation and up-regulation of gene expressions in fibroblasts.
BACKGROUND: Vacuum-assisted closure induces microdeformations of the wound surface and accelerates healing of complex wounds; however, a thorough understanding of the biology of cellular mechanotransduction is lacking. We hypothesized that fibroblast shape and function can be altered in an in vitro vacuum-assisted closure device. METHODS: A 3-dimensional fibrin matrix with cultured murine fibroblasts and an intervening polyurethane foam was exposed to 125 mm Hg suction and compared with similar wells without suction. We measured fibroblast proliferation and morphology using fluorescence microscopy and gene expression change using real-time reverse-transcriptase polymerase chain reaction at 24, 48, and 72 hours. RESULTS: Wells exposed to suction induced significant proliferation of fibroblasts and morphologic changes visible by larger, rounder, and notable dendrite-like branching and process extensions. Type 1 collagen alpha 1 (COL1A1), fibroblast growth factor 2 (FGF2, bFGF), and transforming growth factor beta 1 (TGFβ1) were all up-regulated after 48 hours of exposure to suction. Smooth muscle actin alpha 2 (Acta2, α-SMA) was up-regulated after 72 hours. CONCLUSIONS: Microdeformations produced by the combination of polyurethane foam and suction are associated with increased fibroblast proliferation and up-regulation of gene expressions in fibroblasts.
Authors: Zhen Wang; Kenton D Fong; Toan-Thang Phan; Ivor J Lim; Michael T Longaker; George P Yang Journal: J Cell Physiol Date: 2006-02 Impact factor: 6.384
Authors: Kwonseop Kim; Anna Sirota; Yan-hua Chen Yh; Shiloh B Jones; Ronald Dudek; George W Lanford; Chittam Thakore; Qun Lu Journal: Exp Cell Res Date: 2002-05-01 Impact factor: 3.905
Authors: Shadi Lalezari; Christine J Lee; Anna A Borovikova; Derek A Banyard; Keyianoosh Z Paydar; Garrett A Wirth; Alan D Widgerow Journal: Int Wound J Date: 2016-09-29 Impact factor: 3.315
Authors: Leandra A Barnes; Clement D Marshall; Tripp Leavitt; Michael S Hu; Alessandra L Moore; Jennifer G Gonzalez; Michael T Longaker; Geoffrey C Gurtner Journal: Adv Wound Care (New Rochelle) Date: 2018-02-01 Impact factor: 4.730
Authors: Vijaykumar S Meli; Praveen K Veerasubramanian; Hamza Atcha; Zachary Reitz; Timothy L Downing; Wendy F Liu Journal: J Leukoc Biol Date: 2019-03-12 Impact factor: 4.962
Authors: Shamik Mascharak; Heather E desJardins-Park; Michael F Davitt; Nicholas J Guardino; Geoffrey C Gurtner; Derrick C Wan; Michael T Longaker Journal: Adv Wound Care (New Rochelle) Date: 2021-10-08 Impact factor: 4.947