BACKGROUND AIMS: Fetal membrane from human placenta tissue has been described as a potential source of stem cells. Despite abundant literature on amnion stem cells, there are limited studies on the stem cell properties of chorion-derived stem cells. METHODS: The main aim was to determine the stemness properties of serial-passaged human chorion-derived stem cells (hCDSC). Quantitative polymerase chain reaction (PCR) was performed to reveal the following stemness gene expression in serial-passaged hCDSC: Oct-4, Sox-2, FGF-4, Rex-1, TERT, Nanog (3), Nestin, FZD-9, ABCG-2 and BST-1. Cell growth rate was evaluated from passage (P) 1 until P5. The colony-forming unit-fibroblast (CFU-F) frequency of P3 and P5 cells and multilineage differentiation potential of P5 cells were determined. The immunophenotype of hCDSC was compared using the surface markers CD9, CD31, CD34, CD44, CD45, CD73, CD90, CD117, HLA-ABC and HLA-DR, -DP and -DQ. Immunostaining for trophoblast markers was done on P0, P1, P3 and P5 cells to detect the contamination of trophoblasts in culture, while chromosomal abnormality was screened by cytogenetic analysis of P5 cells. RESULTS: The surface markers for mesenchymal lineage in hCDSC were more highly expressed at P5 compared with P3 and P0, indicating the increased purity of these stem cells after serial passage. Indeed, all the stemness genes except TERT were expressed at P1, P3 and P5 hCDSC. Furthermore, human chorion contained high clonogenic precursors with a 1:30 CFU-F frequency. Successful adipogenic, chondrogenic and osteogenic differentiation demonstrated the multilineage potential of hCDSC. The karyotyping analysis showed hCDSC maintained chromosomal stability after serial passage. CONCLUSIONS: hCDSC retain multipotent potential even at later passages, hence are a promising source for cell therapy in the future.
BACKGROUND AIMS: Fetal membrane from human placenta tissue has been described as a potential source of stem cells. Despite abundant literature on amnion stem cells, there are limited studies on the stem cell properties of chorion-derived stem cells. METHODS: The main aim was to determine the stemness properties of serial-passaged human chorion-derived stem cells (hCDSC). Quantitative polymerase chain reaction (PCR) was performed to reveal the following stemness gene expression in serial-passaged hCDSC: Oct-4, Sox-2, FGF-4, Rex-1, TERT, Nanog (3), Nestin, FZD-9, ABCG-2 and BST-1. Cell growth rate was evaluated from passage (P) 1 until P5. The colony-forming unit-fibroblast (CFU-F) frequency of P3 and P5 cells and multilineage differentiation potential of P5 cells were determined. The immunophenotype of hCDSC was compared using the surface markers CD9, CD31, CD34, CD44, CD45, CD73, CD90, CD117, HLA-ABC and HLA-DR, -DP and -DQ. Immunostaining for trophoblast markers was done on P0, P1, P3 and P5 cells to detect the contamination of trophoblasts in culture, while chromosomal abnormality was screened by cytogenetic analysis of P5 cells. RESULTS: The surface markers for mesenchymal lineage in hCDSC were more highly expressed at P5 compared with P3 and P0, indicating the increased purity of these stem cells after serial passage. Indeed, all the stemness genes except TERT were expressed at P1, P3 and P5 hCDSC. Furthermore, human chorion contained high clonogenic precursors with a 1:30 CFU-F frequency. Successful adipogenic, chondrogenic and osteogenic differentiation demonstrated the multilineage potential of hCDSC. The karyotyping analysis showed hCDSC maintained chromosomal stability after serial passage. CONCLUSIONS: hCDSC retain multipotent potential even at later passages, hence are a promising source for cell therapy in the future.
Authors: Jennifer M Ryan; Allison R Pettit; Pascale V Guillot; Jerry K Y Chan; Nicholas M Fisk Journal: Stem Cell Rev Rep Date: 2013-08 Impact factor: 5.739
Authors: Olga Genbacev; Matthew Donne; Mirhan Kapidzic; Matthew Gormley; Julie Lamb; Jacqueline Gilmore; Nicholas Larocque; Gabriel Goldfien; Tamara Zdravkovic; Michael T McMaster; Susan J Fisher Journal: Stem Cells Date: 2011-09 Impact factor: 6.277
Authors: Haiying Liu; Padma Murthi; Sharon Qin; Gina D Kusuma; Anthony J Borg; Martin Knöfler; Peter Haslinger; Ursula Manuelpillai; Mark D Pertile; Mohamed Abumaree; Bill Kalionis Journal: Reprod Sci Date: 2014-04-01 Impact factor: 3.060
Authors: Branko Skovrlj; Javier Z Guzman; Motasem Al Maaieh; Samuel K Cho; James C Iatridis; Sheeraz A Qureshi Journal: Spine J Date: 2014-06-11 Impact factor: 4.166
Authors: Bo Kyung Koo; In Yang Park; Jiyeon Kim; Ji-Hyun Kim; Ahlm Kwon; Myungshin Kim; Yonggoo Kim; Jong Chul Shin; Jong-Hoon Kim Journal: J Korean Med Sci Date: 2012-07-25 Impact factor: 2.153
Authors: Gemma N Jones; Dafni Moschidou; Tamara-Isabel Puga-Iglesias; Katarzyna Kuleszewicz; Maximilien Vanleene; Sandra J Shefelbine; George Bou-Gharios; Nicholas M Fisk; Anna L David; Paolo De Coppi; Pascale V Guillot Journal: PLoS One Date: 2012-09-04 Impact factor: 3.240