Literature DB >> 21227989

Interpretation of real-time PCR results for hepatitis C virus RNA when viral load is below quantification limits.

Syria Laperche1, Françoise Bouchardeau, Elisabeth André-Garnier, Vincent Thibault, Anne-Marie Roque-Afonso, Pascale Trimoulet, Ronald Colimon, Gilles Duverlie, Hélène Leguillou-Guillemette, Françoise Lunel, Magali Bouvier-Alias, Jean-Michel Pawlotsky, Cécile Henquell, Evelyne Schvoerer, Françoise Stoll-Keller, Marie-Laure Chaix, Michel Branger, Catherine Gaudy-Graffin, Arielle R Rosenberg, Bruno Pozzetto, Sophie Vallet, Yazid Baazia, Jacques Izopet, Jean-Jacques Lefrère.   

Abstract

Hepatitis C virus RNA quantification results obtained in 18 laboratories using real-time PCR methods with 10 negative samples and 22 sample dilutions (viral loads of 0.5 to 500 IU/ml) showed a score of correct results of up to 93.5%. However, 55.6% of the laboratories did not follow the recommendations for the interpretation of their results, leading to ambiguous conclusions.

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Year:  2011        PMID: 21227989      PMCID: PMC3067753          DOI: 10.1128/JCM.01511-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  8 in total

1.  Multilaboratory comparison of hepatitis C virus viral load assays.

Authors:  A M Caliendo; A Valsamakis; Y Zhou; B Yen-Lieberman; J Andersen; S Young; A Ferreira-Gonzalez; G J Tsongalis; R Pyles; J W Bremer; N S Lurain
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

Review 2.  Therapy of hepatitis C: from empiricism to eradication.

Authors:  Jean-Michel Pawlotsky
Journal:  Hepatology       Date:  2006-02       Impact factor: 17.425

3.  Evaluation of the COBAS Hepatitis C Virus (HCV) TaqMan analyte-specific reagent assay and comparison to the COBAS Amplicor HCV Monitor V2.0 and Versant HCV bDNA 3.0 assays.

Authors:  Eric Q Konnick; Sheri M Williams; Edward R Ashwood; David R Hillyard
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

4.  Verification of an assay for quantification of hepatitis C virus RNA by use of an analyte-specific reagent and two different extraction methods.

Authors:  Michael S Forman; Alexandra Valsamakis
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

5.  Experience of mandatory nucleic acid test (NAT) screening across all blood organizations in Germany: NAT yield versus breakthrough transmissions.

Authors:  C Micha Nübling; Margarethe Heiden; Michael Chudy; Julia Kress; Rainer Seitz; Brigitte Keller-Stanislawski; Markus B Funk
Journal:  Transfusion       Date:  2009-05-14       Impact factor: 3.157

6.  Improving the diagnosis of acute hepatitis C virus infection with expanded viral load criteria.

Authors:  Barbara H McGovern; Christopher E Birch; Melinda J Bowen; Laura L Reyor; Ellen H Nagami; Raymond T Chung; Arthur Y Kim
Journal:  Clin Infect Dis       Date:  2009-10-01       Impact factor: 9.079

7.  Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent.

Authors:  James M Barbeau; Jennifer Goforth; Angela M Caliendo; Frederick S Nolte
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

Review 8.  Expert opinion on the treatment of patients with chronic hepatitis C.

Authors:  S Zeuzem; T Berg; B Moeller; H Hinrichsen; S Mauss; H Wedemeyer; C Sarrazin; D Hueppe; E Zehnter; M P Manns
Journal:  J Viral Hepat       Date:  2008-08-28       Impact factor: 3.728

  8 in total
  3 in total

1.  Utility of hepatitis C viral load monitoring on direct-acting antiviral therapy.

Authors:  Sreetha Sidharthan; Anita Kohli; Zayani Sims; Amy Nelson; Anu Osinusi; Henry Masur; Shyam Kottilil
Journal:  Clin Infect Dis       Date:  2015-03-02       Impact factor: 9.079

2.  The predictors of sustained virological response with sofosbuvir and ribavirin in patients with chronic hepatitis C genotype 2.

Authors:  Sung Yong Han; Hyun Young Woo; Jeong Heo; Sang Gyu Park; Sung Ik Pyeon; Young Joo Park; Dong Uk Kim; Gwang Ha Kim; Hyung Hoi Kim; Geun Am Song; Mong Cho
Journal:  Korean J Intern Med       Date:  2019-03-19       Impact factor: 2.884

Review 3.  HCV Detection, Discrimination, and Genotyping Technologies.

Authors:  Shrikant Dashrath Warkad; Satish Balasaheb Nimse; Keum-Soo Song; Taisun Kim
Journal:  Sensors (Basel)       Date:  2018-10-12       Impact factor: 3.576

  3 in total

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