Literature DB >> 21221949

Analysis of cytoplasmic membrane proteome of Streptococcus pneumoniae by shotgun proteomic approach.

Chi-Won Choi1, Sung-Ho Yun, Sang-Oh Kwon, Sun-Hee Leem, Jong-Soon Choi, Chi-Young Yun, Seung Il Kim.   

Abstract

In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferase systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21221949     DOI： 10.1007/s12275-010-0220-9

Source DB:  PubMed          Journal:  J Microbiol        ISSN： 1225-8873            Impact factor:   3.422


  23 in total

1.  The Ami-AliA/AliB permease of Streptococcus pneumoniae is involved in nasopharyngeal colonization but not in invasive disease.

Authors:  A R Kerr; P V Adrian; S Estevão; R de Groot; G Alloing; J-P Claverys; T J Mitchell; P W M Hermans
Journal:  Infect Immun       Date:  2004-07       Impact factor: 3.441

2.  Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein.

Authors:  Yasushi Ishihama; Yoshiya Oda; Tsuyoshi Tabata; Toshitaka Sato; Takeshi Nagasu; Juri Rappsilber; Matthias Mann
Journal:  Mol Cell Proteomics       Date:  2005-06-14       Impact factor: 5.911

3.  A proteomic analysis of penicillin resistance in Streptococcus pneumoniae reveals a novel role for PstS, a subunit of the phosphate ABC transporter.

Authors:  Hafid Soualhine; Vicky Brochu; François Ménard; Barbara Papadopoulou; Karl Weiss; Michel G Bergeron; Danielle Légaré; Jolyne Drummelsmith; Marc Ouellette
Journal:  Mol Microbiol       Date:  2005-12       Impact factor: 3.501

4.  Comparison of extraction procedures for proteome analysis of Streptococcus pneumoniae and a basic reference map.

Authors:  Vesela Encheva; Saheer E Gharbia; Robin Wait; Shajna Begum; Haroun N Shah
Journal:  Proteomics       Date:  2006-06       Impact factor: 3.984

5.  Genome sequence of Avery's virulent serotype 2 strain D39 of Streptococcus pneumoniae and comparison with that of unencapsulated laboratory strain R6.

Authors:  Joel A Lanie; Wai-Leung Ng; Krystyna M Kazmierczak; Tiffany M Andrzejewski; Tanja M Davidsen; Kyle J Wayne; Hervé Tettelin; John I Glass; Malcolm E Winkler
Journal:  J Bacteriol       Date:  2006-10-13       Impact factor: 3.490

6.  Proteomic analysis of the Escherichia coli outer membrane.

Authors:  M P Molloy; B R Herbert; M B Slade; T Rabilloud; A S Nouwens; K L Williams; A A Gooley
Journal:  Eur J Biochem       Date:  2000-05

7.  Complete genome sequence of a virulent isolate of Streptococcus pneumoniae.

Authors:  H Tettelin; K E Nelson; I T Paulsen; J A Eisen; T D Read; S Peterson; J Heidelberg; R T DeBoy; D H Haft; R J Dodson; A S Durkin; M Gwinn; J F Kolonay; W C Nelson; J D Peterson; L A Umayam; O White; S L Salzberg; M R Lewis; D Radune; E Holtzapple; H Khouri; A M Wolf; T R Utterback; C L Hansen; L A McDonald; T V Feldblyum; S Angiuoli; T Dickinson; E K Hickey; I E Holt; B J Loftus; F Yang; H O Smith; J C Venter; B A Dougherty; D A Morrison; S K Hollingshead; C M Fraser
Journal:  Science       Date:  2001-07-20       Impact factor: 47.728

8.  Proteomic analysis of growth phase-dependent proteins of Streptococcus pneumoniae.

Authors:  Kwang-Jun Lee; Song-Mee Bae; Myoung-Ro Lee; Sun-Mi Yeon; Young-Hee Lee; Ki-Sang Kim
Journal:  Proteomics       Date:  2006-02       Impact factor: 3.984

9.  The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9.

Authors:  Rozenn Gardan; Colette Besset; Alain Guillot; Christophe Gitton; Véronique Monnet
Journal:  J Bacteriol       Date:  2009-05-15       Impact factor: 3.490

10.  Analysis of aromatic catabolic pathways in Pseudomonas putida KT 2440 using a combined proteomic approach: 2-DE/MS and cleavable isotope-coded affinity tag analysis.

Authors:  Young Hwan Kim; Kun Cho; Sung-Ho Yun; Jin Young Kim; Kyung-Hoon Kwon; Jong Shin Yoo; Seung Il Kim
Journal:  Proteomics       Date:  2006-02       Impact factor: 3.984
View more
  3 in total

1.  Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker.

Authors:  Chi-Won Choi; Hee-Young An; Yong Ju Lee; Yeol Gyun Lee; Sung Ho Yun; Edmond Changkyun Park; Yeonhee Hong; Gun-Hwa Kim; Jae-Eun Park; Sun Jong Baek; Hyun Sik Kim; Seung Il Kim
Journal:  J Microbiol       Date:  2013-10-31       Impact factor: 3.422

2.  Identification of potential new protein vaccine candidates through pan-surfomic analysis of pneumococcal clinical isolates from adults.

Authors:  Alfonso Olaya-Abril; Irene Jiménez-Munguía; Lidia Gómez-Gascón; Ignacio Obando; Manuel J Rodríguez-Ortega
Journal:  PLoS One       Date:  2013-07-23       Impact factor: 3.240

3.  A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163.

Authors:  María de la Luz Mohedano; Pasquale Russo; Vivian de Los Ríos; Vittorio Capozzi; Pilar Fernández de Palencia; Giuseppe Spano; Paloma López
Journal:  Open Biol       Date:  2014-02-26       Impact factor: 6.411
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.