OBJECTIVE: To determine if keratocyte populations are different in corneas with Fuchs dystrophy compared with control corneas. METHODS: Eleven corneas excised during penetrating keratoplasty for Fuchs dystrophy and 5 control corneas of eyes enucleated for choroidal melanoma were examined using light microscopy. Twenty control corneas age-matched to the corneas with Fuchs dystrophy were examined using confocal microscopy in vivo. The number of keratocytes in a full-thickness column of central stroma with frontal area of 1 mm(2), determined using histologic and confocal methods, was compared between corneas with Fuchs dystrophy and controls. RESULTS: By histology, the mean (SD) number of cells in a full-thickness column of stroma in Fuchs dystrophy (12 215 [1394] cells) was less than in control corneas (15 628 [710] cells; P < .001). The mean (SD) number of keratocytes in the anterior 10% of the stroma of corneas with Fuchs dystrophy (682 [274] cells) was less than in the control corneas measured using histology (1858 [404] cells; P < .001) and confocal microscopy (1481 [397] cells; P < .001). CONCLUSIONS: Keratocytes are depleted by 54% to 63% in the anterior 10% of the stroma of corneas that require penetrating keratoplasty for Fuchs dystrophy. Keratocyte loss might contribute to anterior stromal changes that persist and degrade vision after endothelial keratoplasty.
OBJECTIVE: To determine if keratocyte populations are different in corneas with Fuchs dystrophy compared with control corneas. METHODS: Eleven corneas excised during penetrating keratoplasty for Fuchs dystrophy and 5 control corneas of eyes enucleated for choroidal melanoma were examined using light microscopy. Twenty control corneas age-matched to the corneas with Fuchs dystrophy were examined using confocal microscopy in vivo. The number of keratocytes in a full-thickness column of central stroma with frontal area of 1 mm(2), determined using histologic and confocal methods, was compared between corneas with Fuchs dystrophy and controls. RESULTS: By histology, the mean (SD) number of cells in a full-thickness column of stroma in Fuchs dystrophy (12 215 [1394] cells) was less than in control corneas (15 628 [710] cells; P < .001). The mean (SD) number of keratocytes in the anterior 10% of the stroma of corneas with Fuchs dystrophy (682 [274] cells) was less than in the control corneas measured using histology (1858 [404] cells; P < .001) and confocal microscopy (1481 [397] cells; P < .001). CONCLUSIONS: Keratocytes are depleted by 54% to 63% in the anterior 10% of the stroma of corneas that require penetrating keratoplasty for Fuchs dystrophy. Keratocyte loss might contribute to anterior stromal changes that persist and degrade vision after endothelial keratoplasty.
Authors: A V Ljubimov; R E Burgeson; R J Butkowski; J R Couchman; R R Wu; Y Ninomiya; Y Sado; E Maguen; A B Nesburn; M C Kenney Journal: Invest Ophthalmol Vis Sci Date: 1996-05 Impact factor: 4.799
Authors: L M Schrems-Hoesl; W A Schrems; A Cruzat; B M Shahatit; H A Bayhan; U V Jurkunas; P Hamrah Journal: Eye (Lond) Date: 2012-11-16 Impact factor: 3.775
Authors: Taemi Horikawa; Sara M Thomasy; Amelia A Stanley; Allison S Calderon; Jennifer Li; Lana L Linton; Christopher J Murphy Journal: Cornea Date: 2016-10 Impact factor: 2.651
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