| Literature DB >> 2121897 |
A S Stepanov1, O B Puzanova, O G Loginova, B N Ilyashenko.
Abstract
Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAM beta 1) and Bacillus anthracis plasmid pX02 were introduced into B. anthracis by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 10(3) c.f.u. per micrograms DNA) and allows different cloning vectors with molecular masses ranging from 1.8 to 17.7 MDa to be introduced into B. anthracis.Entities:
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Year: 1990 PMID： 2121897 DOI： 10.1099/00221287-136-7-1217
Source DB: PubMed Journal: J Gen Microbiol ISSN： 0022-1287