Literature DB >> 21216984

Replacement of the 3' untranslated variable region of mosquito-borne dengue virus with that of tick-borne Langat virus does not alter vector specificity.

Ebenezer Tumban1, Dana N Mitzel, Nyree E Maes, Christopher T Hanson, Stephen S Whitehead, Kathryn A Hanley.   

Abstract

The four major flavivirus clades are transmitted by mosquitoes, ticks, directly between vertebrates or directly between arthropods, respectively, but the molecular determinants of mode of transmission in flaviviruses are unknown. To assess the role of the UTRs in transmission, we generated chimeric genomes in which the 5' UTR, capsid and/or 3' UTR of mosquito-borne dengue virus serotype 4 (rDENV-4) were replaced, separately or in combination, with those of tick-borne Langat virus (rLGTV). None of the chimeric genomes yielded detectable virus following transfection. Replacement of the variable region (VR) in the rDENV-4 3' UTR with that of rLGTV generated virus rDENV-4-rLGTswapVR, which showed lower replication than its wild-type parents in mammalian but not mosquito cells in culture and was able to infect mosquitoes in vivo. Neither rDENV-4 nor rDENV-4-rLGTswapVR could infect larval Ixodes scapularis ticks immersed in virus, while rLGTV was highly infectious via this route.

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Year:  2011        PMID： 21216984      PMCID： PMC3133702          DOI： 10.1099/vir.0.026997-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  61 in total

1.  A live attenuated recombinant dengue-4 virus vaccine candidate with restricted capacity for dissemination in mosquitoes and lack of transmission from vaccinees to mosquitoes.

Authors:  J M Troyer; K A Hanley; S S Whitehead; D Strickman; R A Karron; A P Durbin; B R Murphy
Journal:  Am J Trop Med Hyg       Date:  2001-11       Impact factor: 2.345

2.  Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus.

Authors:  M B Crabtree; R C Sang; V Stollar; L M Dunster; B R Miller
Journal:  Arch Virol       Date:  2003-06       Impact factor: 2.574

3.  Mfold web server for nucleic acid folding and hybridization prediction.

Authors:  Michael Zuker
Journal:  Nucleic Acids Res       Date:  2003-07-01       Impact factor: 16.971

4.  Complete genome sequence of Montana Myotis leukoencephalitis virus, phylogenetic analysis and comparative study of the 3' untranslated region of flaviviruses with no known vector.

Authors:  Nathalie Charlier; Pieter Leyssen; Cornelis W A Pleij; Philippe Lemey; Frédérique Billoir; Kristel Van Laethem; Anne-Mieke Vandamme; Erik De Clercq; Xavier de Lamballerie; Johan Neyts
Journal:  J Gen Virol       Date:  2002-08       Impact factor: 3.891

5.  Paired charge-to-alanine mutagenesis of dengue virus type 4 NS5 generates mutants with temperature-sensitive, host range, and mouse attenuation phenotypes.

Authors:  Kathryn A Hanley; Jay J Lee; Joseph E Blaney; Brian R Murphy; Stephen S Whitehead
Journal:  J Virol       Date:  2002-01       Impact factor: 5.103

6.  West Nile virus/dengue type 4 virus chimeras that are reduced in neurovirulence and peripheral virulence without loss of immunogenicity or protective efficacy.

Authors:  Alexander G Pletnev; Robert Putnak; Jim Speicher; Eric J Wagar; David W Vaughn
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-05       Impact factor: 11.205

7.  Complete genome sequence, taxonomic assignment, and comparative analysis of the untranslated regions of the Modoc virus, a flavivirus with no known vector.

Authors:  Pieter Leyssen; Nathalie Charlier; Philippe Lemey; Frédérique Billoir; Anne-Mieke Vandamme; Erik De Clercq; Xavier de Lamballerie; Johan Neyts
Journal:  Virology       Date:  2002-02-01       Impact factor: 3.616

8.  Mutations which enhance the replication of dengue virus type 4 and an antigenic chimeric dengue virus type 2/4 vaccine candidate in Vero cells.

Authors:  Joseph E Blaney; Gracielle G Manipon; Cai Yen Firestone; Daniel H Johnson; Christopher T Hanson; Brian R Murphy; Stephen S Whitehead
Journal:  Vaccine       Date:  2003-10-01       Impact factor: 3.641

9.  A trade-off in replication in mosquito versus mammalian systems conferred by a point mutation in the NS4B protein of dengue virus type 4.

Authors:  Kathryn A Hanley; Luella R Manlucu; Lara E Gilmore; Joseph E Blaney; Christopher T Hanson; Brian R Murphy; Stephen S Whitehead
Journal:  Virology       Date:  2003-07-20       Impact factor: 3.616

10.  Isolation of a new flavivirus related to cell fusing agent virus (CFAV) from field-collected flood-water Aedes mosquitoes sampled from a dambo in central Kenya.

Authors:  R C Sang; A Gichogo; J Gachoya; M D Dunster; V Ofula; A R Hunt; M B Crabtree; B R Miller; L M Dunster
Journal:  Arch Virol       Date:  2003-06       Impact factor: 2.574
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  8 in total

1.  Structural gene (prME) chimeras of St Louis encephalitis virus and West Nile virus exhibit altered in vitro cytopathic and growth phenotypes.

Authors:  Payal D Maharaj; Michael Anishchenko; Stanley A Langevin; Ying Fang; William K Reisen; Aaron C Brault
Journal:  J Gen Virol       Date:  2011-09-21       Impact factor: 3.891

Review 2.  RNA Structure Duplications and Flavivirus Host Adaptation.

Authors:  Sergio M Villordo; Juan M Carballeda; Claudia V Filomatori; Andrea V Gamarnik
Journal:  Trends Microbiol       Date:  2016-02-03       Impact factor: 17.079

3.  Replacement of conserved or variable sequences of the mosquito-borne dengue virus 3' UTR with homologous sequences from Modoc virus does not change infectivity for mosquitoes.

Authors:  Ebenezer Tumban; Nyree E Maes; Erin E Schirtzinger; Katherine I Young; Christopher T Hanson; Stephen S Whitehead; Kathryn A Hanley
Journal:  J Gen Virol       Date:  2012-12-19       Impact factor: 3.891

4.  Dengue virus RNA structure specialization facilitates host adaptation.

Authors:  Sergio M Villordo; Claudia V Filomatori; Irma Sánchez-Vargas; Carol D Blair; Andrea V Gamarnik
Journal:  PLoS Pathog       Date:  2015-01-30       Impact factor: 6.823

5.  Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells.

Authors:  Rungrat Saiyasombat; Jimena Carrillo-Tripp; Wyatt Allen Miller; Peter J Bredenbeek; Bradley J Blitvich
Journal:  Virol J       Date:  2014-08-24       Impact factor: 4.099

6.  Possible Roles of New Mutations Shared by Asian and American Zika Viruses.

Authors:  Shozo Yokoyama; William T Starmer
Journal:  Mol Biol Evol       Date:  2017-03-01       Impact factor: 16.240

Review 7.  Flavivirus reverse genetic systems, construction techniques and applications: a historical perspective.

Authors:  Fabien Aubry; Antoine Nougairède; Ernest A Gould; Xavier de Lamballerie
Journal:  Antiviral Res       Date:  2014-12-12       Impact factor: 5.970

8.  Induction and suppression of tick cell antiviral RNAi responses by tick-borne flaviviruses.

Authors:  Esther Schnettler; Hana Tykalová; Mick Watson; Mayuri Sharma; Mark G Sterken; Darren J Obbard; Samuel H Lewis; Melanie McFarlane; Lesley Bell-Sakyi; Gerald Barry; Sabine Weisheit; Sonja M Best; Richard J Kuhn; Gorben P Pijlman; Margo E Chase-Topping; Ernest A Gould; Libor Grubhoffer; John K Fazakerley; Alain Kohl
Journal:  Nucleic Acids Res       Date:  2014-07-22       Impact factor: 16.971
  8 in total

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