Literature DB >> 2121618

High-efficiency transformation of Listeria monocytogenes by electroporation of penicillin-treated cells.

S F Park1, G S Stewart.   

Abstract

A procedure has been developed for electroporation-mediated transformation of Listeria monocytogenes with plasmid DNA. The method was optimized for intact cells of L. monocytogenes 23074 by determining the effects of field strength, cell density, and plasmid DNA topology. Transformation efficiencies were dramatically increased when cells were treated with penicillin. Optimum frequencies of transformation (4 x 10(6) transformants/microgram DNA) were obtained when cells were grown in 10 micrograms/ml of penicillin G and electroporated at a field strength of 10 kV/cm. Using this procedure, transformation of relaxed plasmid DNA from ligation reactions provided 1 x 10(4) transformants/microgram DNA, allowing direct molecular cloning of DNA into this organism.

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Year:  1990        PMID： 2121618     DOI： 10.1016/0378-1119(90)90479-b

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  120 in total

1.  Mycobacterial infection of macrophages results in membrane-permeable phagosomes.

Authors:  R Teitelbaum; M Cammer; M L Maitland; N E Freitag; J Condeelis; B R Bloom
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

2.  Induction of human immunodeficiency virus (HIV)-specific CD8 T-cell responses by Listeria monocytogenes and a hyperattenuated Listeria strain engineered to express HIV antigens.

Authors:  R S Friedman; F R Frankel; Z Xu; J Lieberman
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

3.  Identification and disruption of BetL, a secondary glycine betaine transport system linked to the salt tolerance of Listeria monocytogenes LO28.

Authors:  R D Sleator; C G Gahan; T Abee; C Hill
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

4.  Broad-host-range shuttle vectors for screening of regulated promoter activity in viridans group streptococci: isolation of a pH-regulated promoter.

Authors:  A J Vriesema; R Brinkman; J Kok; J Dankert; S A Zaat
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

Review 5.  Molecular determinants of Listeria monocytogenes pathogenesis.

Authors:  D A Portnoy; T Chakraborty; W Goebel; P Cossart
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441

6.  pbp2229-mediated nisin resistance mechanism in Listeria monocytogenes confers cross-protection to class IIa bacteriocins and affects virulence gene expression.

Authors:  Anne Gravesen; Birgitte Kallipolitis; Kim Holmstrøm; Poul Erik Høiby; Manilduth Ramnath; Susanne Knøchel
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

7.  Role of FliF and FliI of Listeria monocytogenes in flagellar assembly and pathogenicity.

Authors:  Armelle Bigot; Hélène Pagniez; Eléonore Botton; Claude Fréhel; Iharilalao Dubail; Christine Jacquet; Alain Charbit; Catherine Raynaud
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

8.  Pathogenicity and immunogenicity of a vaccine strain of Listeria monocytogenes that relies on a suicide plasmid to supply an essential gene product.

Authors:  Xinyan Zhao; Zhongxia Li; Baiyan Gu; Fred R Frankel
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

9.  Novel activator of mannose-specific phosphotransferase system permease expression in Listeria innocua, identified by screening for pediocin AcH resistance.

Authors:  Junfeng Xue; Ian Hunter; Tori Steinmetz; Adam Peters; Bibek Ray; Kurt W Miller
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

Review 10.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316
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