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Literature DB >> 2121587

High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.

Abstract

High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.

Entities: Chemical Species

Mesh：

Substances：
DNA, Bacterial

Year: 1990 PMID： 2121587 DOI： 10.1111/j.1574-6968.1990.tb13982.x

Source DB: PubMed Journal: FEMS Microbiol Lett ISSN： 0378-1097 Impact factor: 2.742

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Cited

45 in total

1. Nitric oxide signaling and transcriptional control of denitrification genes in Pseudomonas stutzeri.

Authors: K U Vollack; W G Zumft
Journal: J Bacteriol Date: 2001-04 Impact factor: 3.490

2. Transcriptional control of the hydrogen cyanide biosynthetic genes hcnABC by the anaerobic regulator ANR and the quorum-sensing regulators LasR and RhlR in Pseudomonas aeruginosa.

Authors: G Pessi; D Haas
Journal: J Bacteriol Date: 2000-12 Impact factor: 3.490

3. PchC thioesterase optimizes nonribosomal biosynthesis of the peptide siderophore pyochelin in Pseudomonas aeruginosa.

Authors: Cornelia Reimmann; Hiten M Patel; Christopher T Walsh; Dieter Haas
Journal: J Bacteriol Date: 2004-10 Impact factor: 3.490

4. Role of the Tat ransport system in nitrous oxide reductase translocation and cytochrome cd1 biosynthesis in Pseudomonas stutzeri.

Authors: M P Heikkilä; U Honisch; P Wunsch; W G Zumft
Journal: J Bacteriol Date: 2001-03 Impact factor: 3.490

5. GacA-controlled activation of promoters for small RNA genes in Pseudomonas fluorescens.

Authors: Bérénice Humair; Birgit Wackwitz; Dieter Haas
Journal: Appl Environ Microbiol Date: 2010-01-04 Impact factor: 4.792

6. Identification and functional characterization of an ABC transport system involved in polysaccharide export of A-band lipopolysaccharide in Pseudomonas aeruginosa.

Authors: H L Rocchetta; J S Lam
Journal: J Bacteriol Date: 1997-08 Impact factor: 3.490

High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.

1. Nitric oxide signaling and transcriptional control of denitrification genes in Pseudomonas stutzeri.

2. Transcriptional control of the hydrogen cyanide biosynthetic genes hcnABC by the anaerobic regulator ANR and the quorum-sensing regulators LasR and RhlR in Pseudomonas aeruginosa.

3. PchC thioesterase optimizes nonribosomal biosynthesis of the peptide siderophore pyochelin in Pseudomonas aeruginosa.

4. Role of the Tat ransport system in nitrous oxide reductase translocation and cytochrome cd1 biosynthesis in Pseudomonas stutzeri.

5. GacA-controlled activation of promoters for small RNA genes in Pseudomonas fluorescens.

6. Identification and functional characterization of an ABC transport system involved in polysaccharide export of A-band lipopolysaccharide in Pseudomonas aeruginosa.

7. Functional domains of NosR, a novel transmembrane iron-sulfur flavoprotein necessary for nitrous oxide respiration.

8. Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum sensing.

9. Stereospecificity of the siderophore pyochelin outer membrane transporters in fluorescent pseudomonads.

10. Plant lectin-like bacteriocin from a rhizosphere-colonizing Pseudomonas isolate.