OBJECTIVE: The function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays. METHODS: Powdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H(3)PO(4) for 10min or (2) untreated (control). The presence of MMP-3 was measured using a colorimetric assay system (QuantiSir™, Epigentek, USA). Additional cryo-fractured dentine fragments were processed for immunohistochemical identification of MMP-3 under FEI-SEM. Casein-zymography was used to investigate MMP-3 activity. RESULTS: MMP-3 detected level was 2.732ng/μL in partially demineralised dentine powder, whilst it increased to 3.280ng/μL in mineralised dentine. The FEI-SEM analysis revealed positive immunolabelling patterns for MMP-3, predominantly localized on the intertubular collagen fibrillar network showing MMP-3 directly or indirectly bound to the collagen fibrils. Casein-zymograms showed positive proteolytic activity for MMP-3 in demineralised dentine powder. CONCLUSION: The results of the study clearly revealed the presence and distribution of MMP3 in human sound dentine. Whilst the presence was verified, its role is still unclear. Future studies are needed to investigate the possible involvement of MMP-3 in physiological and pathological condition of the dentine-pulp complex.
OBJECTIVE: The function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays. METHODS: Powdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H(3)PO(4) for 10min or (2) untreated (control). The presence of MMP-3 was measured using a colorimetric assay system (QuantiSir™, Epigentek, USA). Additional cryo-fractured dentine fragments were processed for immunohistochemical identification of MMP-3 under FEI-SEM. Casein-zymography was used to investigate MMP-3 activity. RESULTS:MMP-3 detected level was 2.732ng/μL in partially demineralised dentine powder, whilst it increased to 3.280ng/μL in mineralised dentine. The FEI-SEM analysis revealed positive immunolabelling patterns for MMP-3, predominantly localized on the intertubular collagen fibrillar network showing MMP-3 directly or indirectly bound to the collagen fibrils. Casein-zymograms showed positive proteolytic activity for MMP-3 in demineralised dentine powder. CONCLUSION: The results of the study clearly revealed the presence and distribution of MMP3 in human sound dentine. Whilst the presence was verified, its role is still unclear. Future studies are needed to investigate the possible involvement of MMP-3 in physiological and pathological condition of the dentine-pulp complex.
Authors: Yoshihiro Nishitani; Masahiro Yoshiyama; Bakul Wadgaonkar; Lorenzo Breschi; Ferdinando Mannello; Annalisa Mazzoni; Ricardo M Carvalho; Leo Tjäderhane; Franklin R Tay; David H Pashley Journal: Eur J Oral Sci Date: 2006-04 Impact factor: 2.612
Authors: A Mazzoni; F D Nascimento; M Carrilho; I Tersariol; V Papa; L Tjäderhane; R Di Lenarda; F R Tay; D H Pashley; L Breschi Journal: J Dent Res Date: 2012-02-21 Impact factor: 6.116
Authors: A Mazzoni; F M Apolonio; V P A Saboia; S Santi; V Angeloni; V Checchi; R Curci; R Di Lenarda; F R Tay; D H Pashley; L Breschi Journal: J Dent Res Date: 2013-12-11 Impact factor: 6.116
Authors: Martha Goël Brackett; Kelli A Agee; William W Brackett; William O Key; Camila Sabatini; Melissa T Kato; Marilia A R Buzalaf; Leo Tjäderhane; David H Pashley Journal: J Nat Sci Date: 2015-06
Authors: A Mazzoni; L Tjäderhane; V Checchi; R Di Lenarda; T Salo; F R Tay; D H Pashley; L Breschi Journal: J Dent Res Date: 2014-12-22 Impact factor: 6.116
Authors: D L S Scheffel; C C Delgado; D G Soares; F G Basso; C A de Souza Costa; D H Pashley; J Hebling Journal: Oper Dent Date: 2015-03-12 Impact factor: 2.440
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela R Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2012-08-16 Impact factor: 5.304