OBJECTIVE: In order to improving the existent breeds of Fritillaria cirrhosa, increasing its medicinal ingredients and enriching the resources of heredity breeding. METHODS: By using of low-temperature stratification treatment, the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology. The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared. RESULT: Detected the plant morphology and chromosome number, it is shown that the induced material obviously possessed the characteristics of polyploid. CONCLUSION: With the treatment of 30 mg/L GA3 for 32 h and stratification for 70 d, the seed germination rate of Fritillaria cirrhosa reached 67.0%. After treated with 0.1% colchicine solution for 48 h, the stratificationed mature seed showed polyploid inductivity of 85.7%.
OBJECTIVE: In order to improving the existent breeds of Fritillaria cirrhosa, increasing its medicinal ingredients and enriching the resources of heredity breeding. METHODS: By using of low-temperature stratification treatment, the seed of Fritillaria cirrhosa completed its after-ripening of physiology and morphology. The induction treatment by different concentrations of colchicine solution and different treatment times for Fritillaria cirrhosa seeds were compared. RESULT: Detected the plant morphology and chromosome number, it is shown that the induced material obviously possessed the characteristics of polyploid. CONCLUSION: With the treatment of 30 mg/L GA3 for 32 h and stratification for 70 d, the seed germination rate of Fritillaria cirrhosa reached 67.0%. After treated with 0.1% colchicine solution for 48 h, the stratificationed mature seed showed polyploid inductivity of 85.7%.