Literature DB >> 21209170

Comparison of two Mycoplasma genitalium real-time PCR detection methodologies.

Jimmy Twin1, Nicole Taylor, Suzanne M Garland, Jane S Hocking, Jennifer Walker, Catriona S Bradshaw, Christopher K Fairley, Sepehr N Tabrizi.   

Abstract

Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).

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Year:  2011        PMID： 21209170      PMCID： PMC3067702          DOI： 10.1128/JCM.02328-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Mycoplasma genitalium PCR: does freezing of specimens affect sensitivity?

Authors:  Katrina Hutton Carlsen; Jorgen Skov Jensen
Journal:  J Clin Microbiol       Date:  2010-08-18       Impact factor: 5.948

2.  The minimal gene complement of Mycoplasma genitalium.

Authors:  C M Fraser; J D Gocayne; O White; M D Adams; R A Clayton; R D Fleischmann; C J Bult; A R Kerlavage; G Sutton; J M Kelley; R D Fritchman; J F Weidman; K V Small; M Sandusky; J Fuhrmann; D Nguyen; T R Utterback; D M Saudek; C A Phillips; J M Merrick; J F Tomb; B A Dougherty; K F Bott; P C Hu; T S Lucier; S N Peterson; H O Smith; C A Hutchison; J C Venter
Journal:  Science       Date:  1995-10-20       Impact factor: 47.728

3.  The measurement of observer agreement for categorical data.

Authors:  J R Landis; G G Koch
Journal:  Biometrics       Date:  1977-03       Impact factor: 2.571

4.  Diagnostic tests. 1: Sensitivity and specificity.

Authors:  D G Altman; J M Bland
Journal:  BMJ       Date:  1994-06-11

5.  Quantitative detection of Mycoplasma genitalium from first-pass urine of men with urethritis and asymptomatic men by real-time PCR.

Authors:  Takashi Yoshida; Takashi Deguchi; Masayasu Ito; Shin-Ichi Maeda; Masayoshi Tamaki; Hiroaki Ishiko
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

6.  Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic.

Authors:  Jørgen Skov Jensen; Eva Björnelius; Birthe Dohn; Peter Lidbrink
Journal:  Sex Transm Dis       Date:  2004-08       Impact factor: 2.830

Review 7.  Mycoplasma genitalium: the aetiological agent of urethritis and other sexually transmitted diseases.

Authors:  Jørgen Skov Jensen
Journal:  J Eur Acad Dermatol Venereol       Date:  2004-01       Impact factor: 6.166

8.  Determination of infectious load of Mycoplasma genitalium in clinical samples of human vaginal cells.

Authors:  Mark W Blaylock; Oxana Musatovova; Janet G Baseman; Joel B Baseman
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

9.  Use of TaqMan 5' nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic.

Authors:  Jørgen Skov Jensen; Eva Björnelius; Birthe Dohn; Peter Lidbrink
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

10.  Detection and quantification of Mycoplasma genitalium in male patients with urethritis.

Authors:  Nicolas Dupin; Gérard Bijaoui; Michaël Schwarzinger; Pauline Ernault; Philippe Gerhardt; Randa Jdid; Salim Hilab; Christina Pantoja; Marc Buffet; Jean-Paul Escande; Jean-Marc Costa
Journal:  Clin Infect Dis       Date:  2003-07-30       Impact factor: 9.079
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  10 in total

1.  Evaluation of the SpeeDx ResistancePlus MG Diagnostic Test for Mycoplasma genitalium on the Applied Biosystems 7500 Fast Quantitative PCR Platform.

Authors:  Jenny P Su; Lit Y Tan; Suzanne M Garland; Sepehr N Tabrizi; Elisa Mokany; Samantha Walker; Catriona S Bradshaw; Tim Read; Gerald L Murray
Journal:  J Clin Microbiol       Date:  2017-12-26       Impact factor: 5.948

2.  Evaluation of the Hologic Panther Transcription-Mediated Amplification Assay for Detection of Mycoplasma genitalium.

Authors:  S N Tabrizi; A M Costa; J Su; P Lowe; C S Bradshaw; C K Fairley; S M Garland
Journal:  J Clin Microbiol       Date:  2016-06-15       Impact factor: 5.948

3.  Analysis of Infection Loads in Mycoplasma genitalium Clinical Specimens by Use of a Commercial Diagnostic Test.

Authors:  Gerald L Murray; Jennifer Danielewski; Kaveesha Bodiyabadu; Dorothy A Machalek; Catriona S Bradshaw; Anna-Maria Costa; Josh Birnie; Suzanne M Garland
Journal:  J Clin Microbiol       Date:  2019-08-26       Impact factor: 5.948

4.  Transmission and selection of macrolide resistant Mycoplasma genitalium infections detected by rapid high resolution melt analysis.

Authors:  Jimmy Twin; Jorgen S Jensen; Catriona S Bradshaw; Suzanne M Garland; Christopher K Fairley; Lim Yi Min; Sepehr N Tabrizi
Journal:  PLoS One       Date:  2012-04-20       Impact factor: 3.240

5.  'The difference in determinants of Chlamydia trachomatis and Mycoplasma genitalium in a sample of young Australian women'.

Authors:  Jennifer Walker; Christopher K Fairley; Catriona S Bradshaw; Sepehr N Tabrizi; Marcus Y Chen; Jimmy Twin; Nicole Taylor; Basil Donovan; John K Kaldor; Kathleen McNamee; Eve Urban; Sandra Walker; Marian Currie; Hudson Birden; Francis Bowden; Jane Gunn; Marie Pirotta; Lyle Gurrin; Veerakathy Harindra; Suzanne Garland; Jane S Hocking
Journal:  BMC Infect Dis       Date:  2011-02-01       Impact factor: 3.090

6.  Multiplex Assay for Simultaneous Detection of Mycoplasma genitalium and Macrolide Resistance Using PlexZyme and PlexPrime Technology.

Authors:  Sepehr N Tabrizi; Lit Y Tan; Samantha Walker; Jimmy Twin; Marin Poljak; Catriona S Bradshaw; Christopher K Fairley; Melanie Bissessor; Elisa Mokany; Alison V Todd; Suzanne M Garland
Journal:  PLoS One       Date:  2016-06-06       Impact factor: 3.240

7.  Use of Pristinamycin for Macrolide-Resistant Mycoplasma genitalium Infection.

Authors:  Tim R H Read; Jørgen S Jensen; Christopher K Fairley; Mieken Grant; Jennifer A Danielewski; Jenny Su; Gerald L Murray; Eric P F Chow; Karen Worthington; Suzanne M Garland; Sepehr N Tabrizi; Catriona S Bradshaw
Journal:  Emerg Infect Dis       Date:  2018-02       Impact factor: 6.883

8.  Mycoplasma genitalium Infection in Adults Reporting Sexual Contact with Infected Partners, Australia, 2008-2016.

Authors:  Josephine B Slifirski; Lenka A Vodstrcil; Christopher K Fairley; Jason J Ong; Eric P F Chow; Marcus Y Chen; Timothy R H Read; Catriona S Bradshaw
Journal:  Emerg Infect Dis       Date:  2017-11       Impact factor: 6.883

9.  Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance.

Authors:  S N Tabrizi; J Su; C S Bradshaw; C K Fairley; S Walker; L Y Tan; E Mokany; S M Garland
Journal:  J Clin Microbiol       Date:  2017-04-05       Impact factor: 5.948

10.  Isolation of Mycoplasma genitalium from endocervical swabs of infertile women.

Authors:  Dunia A Alfarraj; Ali M Somily
Journal:  Saudi Med J       Date:  2017-05       Impact factor: 1.484
  10 in total

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