| Literature DB >> 21208897 |
Johanna Ulvila1, Leena-Maija Vanha-aho, Anni Kleino, Mari Vähä-Mäkilä, Milka Vuoksio, Sinikka Eskelinen, Dan Hultmark, Christine Kocks, Mikko Hallman, Mataleena Parikka, Mika Rämet.
Abstract
Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.Entities:
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Year: 2011 PMID： 21208897 DOI： 10.1189/jlb.0410195
Source DB: PubMed Journal: J Leukoc Biol ISSN： 0741-5400 Impact factor: 4.962