| Literature DB >> 2120801 |
R H Purdy1, P H Moore, P N Rao, N Hagino, T Yamaguchi, P Schmidt, D R Rubinow, A L Morrow, S M Paul.
Abstract
A radioimmunoassay for measuring 3 alpha-hydroxy-5 alpha-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3 alpha-hydroxy-20-oxo-5 alpha-pregnan-11 alpha-yl carboxymethyl ether coupled to bovine serum albumin. 3 alpha-Hydroxy-5 alpha-pregnan-20-one was purified from either extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3 alpha-Hydroxy-5 alpha-pregnan-20-one was detected in plasma from female rats on the day of estrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.Entities:
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Year: 1990 PMID： 2120801 DOI： 10.1016/0039-128x(90)90031-6
Source DB: PubMed Journal: Steroids ISSN： 0039-128X Impact factor: 2.668