| Literature DB >> 2120717 |
M Sugiyama1, H Nomura, O Nimi.
Abstract
We have constructed a promoter-probe vector utilizing the expression of a promoter-less tyrosinase derived from Streptomyces plasmid pIJ702. The vector, pMX100, has single sites for EcoRI, KpnI, BamHI, XbaI, SalI, and SphI for cloning promoter sequences. When the tac promoter was inserted into pMX100, E. coli harboring the chimeric plasmid produced the melanin pigment.Entities:
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Year: 1990 PMID： 2120717 DOI： 10.1016/0147-619x(90)90055-h
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466