Literature DB >> 21205857

Labeling nuclear DNA with hoechst 33342.

Brad Chazotte.   

Abstract

A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells. One advantage of Hoechst 33342 is that it is membrane permeant and, thus, can stain live cells. Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21205857     DOI： 10.1101/pdb.prot5557

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  21 in total

1.  Clinically Compatible Fluorescence Microscopy Based on Moxifloxacin Antibiotic.

Authors:  Seunghoon Lee; Ki Hean Kim
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

2.  Development of a stretch-induced neurotrauma model for medium-throughput screening in vitro: identification of rifampicin as a neuroprotectant.

Authors:  Isabel López-García; Domokos Gerő; Bartosz Szczesny; Petra Szoleczky; Gabor Olah; Katalin Módis; Kangling Zhang; Jungling Gao; Ping Wu; Lawrence C Sowers; Doug DeWitt; Donald S Prough; Csaba Szabo
Journal:  Br J Pharmacol       Date:  2016-11-15       Impact factor: 8.739

3.  Probes for Fluorescent Visualization of Specific Cellular Organelles.

Authors:  Timothy Paul Foster
Journal:  Methods Mol Biol       Date:  2022

4.  Encoding with a fluorescence-activating and absorption-shifting tag generates living bacterial probes for mammalian microbiota imaging.

Authors:  Zhenping Cao; Lu Wang; Rui Liu; Sisi Lin; Feng Wu; Jinyao Liu
Journal:  Mater Today Bio       Date:  2022-06-06

5.  Autoimmunity in Human CE: Correlative with The Fertility Status of The CE Cyst.

Authors:  E A El Saftawy; A Abdelraouf; M A Elsalam; P Zakareya; A Fouad; E A Albadawi; A H S Abobakr Ali; N M Amin
Journal:  Helminthologia       Date:  2022-05-04       Impact factor: 1.176

6.  Salinomycin efficiency assessment in non-tumor (HB4a) and tumor (MCF-7) human breast cells.

Authors:  Andressa Megumi Niwa; Gláucia Fernanda Rocha D Epiro; Lilian Areal Marques; Simone Cristine Semprebon; Daniele Sartori; Lúcia Regina Ribeiro; Mário Sérgio Mantovani
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2016-03-02       Impact factor: 3.000

7.  Puf3 participates in ribosomal biogenesis in malaria parasites.

Authors:  Xiaoying Liang; Kevin J Hart; Gang Dong; Faiza A Siddiqui; Aswathy Sebastian; Xiaolian Li; Istvan Albert; Jun Miao; Scott E Lindner; Liwang Cui
Journal:  J Cell Sci       Date:  2018-03-26       Impact factor: 5.285

8.  MiR-132-3p serves as a tumor suppressor in mantle cell lymphoma via directly targeting SOX11.

Authors:  Baoyu Wu; Jingyu Li; Han Wang; Qian Wu; Hui Liu
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2020-02-10       Impact factor: 3.000

9.  Pro-necrotic Activity of Cationic Mastoparan Peptides in Human Glioblastoma Multiforme Cells Via Membranolytic Action.

Authors:  Annielle Mendes Brito da Silva; Laíz Costa Silva-Gonçalves; Fernando Augusto Oliveira; Manoel Arcisio-Miranda
Journal:  Mol Neurobiol       Date:  2017-09-30       Impact factor: 5.590

10.  Complexity in the binding of minor groove agents: netropsin has two thermodynamically different DNA binding modes at a single site.

Authors:  Edwin A Lewis; Manoj Munde; Shuo Wang; Michael Rettig; Vu Le; Venkata Machha; W David Wilson
Journal:  Nucleic Acids Res       Date:  2011-09-03       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.