Literature DB >> 21205854

Mounting live cells onto microscope slides.

Brad Chazotte.   

Abstract

This protocol describes the mounting of cells which have been grown on cleaned coverslips in cell culture dishes containing the appropriate medium and supplements. Labeling is performed with the coverslips placed in culture dishes. After cells are labeled, the coverslips can be mounted onto microscope slides to be viewed for short-term observation of live cells or longer-term observation of fixed cells. For viewing live cells for longer periods of time, specialized chambers should be used in which suitable growth conditions can be maintained.

Mesh:

Year:  2011        PMID: 21205854     DOI: 10.1101/pdb.prot5554

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  7 in total

1.  Chrysin protects cardiac H9c2 cells against H2O2-induced endoplasmic reticulum stress by up-regulating the Nrf2/PERK pathway.

Authors:  Subramani Yuvaraj; Arumugam Kalaiselvi Ajeeth; Shanavas Syed Mohamed Puhari; Albert Abhishek; Tharmarajan Ramprasath; Varadaraj Vasudevan; Narasimman Vignesh; Govindan Sadasivam Selvam
Journal:  Mol Cell Biochem       Date:  2022-08-09       Impact factor: 3.842

2.  Compound Shape Effects in Minor Groove Binding Affinity and Specificity for Mixed Sequence DNA.

Authors:  Pu Guo; Abdelbasset A Farahat; Ananya Paul; Narinder K Harika; David W Boykin; W David Wilson
Journal:  J Am Chem Soc       Date:  2018-10-24       Impact factor: 15.419

3.  Traction microscopy to identify force modulation in subresolution adhesions.

Authors:  Sangyoon J Han; Youbean Oak; Alex Groisman; Gaudenz Danuser
Journal:  Nat Methods       Date:  2015-06-01       Impact factor: 28.547

4.  Autophagy of germ-granule components, PGL-1 and PGL-3, contributes to DNA damage-induced germ cell apoptosis in C. elegans.

Authors:  Hyemin Min; Yong-Uk Lee; Yhong-Hee Shim; Ichiro Kawasaki
Journal:  PLoS Genet       Date:  2019-05-24       Impact factor: 5.917

5.  Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched Hyphae of an Oomycete Pathogen.

Authors:  Edouard Evangelisti; Liron Shenhav; Temur Yunusov; Marie Le Naour-Vernet; Philipp Rink; Sebastian Schornack
Journal:  mBio       Date:  2019-10-01       Impact factor: 7.867

6.  Synthetic Antibody Mimics Based on Cancer-Targeting Immunostimulatory Peptides.

Authors:  Dante Descalzi-Montoya; Rachel A Montel; Keith Smith; Eugenia Dziopa; Andrieh Darwich; Zheng Yang; Constantine Bitsaktsis; Robert Korngold; David Sabatino
Journal:  Chembiochem       Date:  2020-11-02       Impact factor: 3.164

7.  Protective effect of p-coumaric acid against doxorubicin induced toxicity in H9c2 cardiomyoblast cell lines.

Authors:  Sunitha M Chacko; Kottayath G Nevin; R Dhanyakrishnan; B Prakash Kumar
Journal:  Toxicol Rep       Date:  2015-08-10
  7 in total

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