Literature DB >> 2120109

Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C.

J Blanco1, M Blanco, E A González, M P Alonso, J I Garabal.   

Abstract

Necrotizing Escherichia coli (NTEC) strains grown in the presence of mitomycin C released cell associated necrotizing factors CNF1 and CNF2 to culture medium. Using culture filtrates from 96 mitomycin C treated E. coli strains, we have found that a modified HeLa cell assay was a more sensitive and specific method for the detection of CNF1 and CNF2 than the Vero cell assay and the rabbit skin test.

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Year:  1990        PMID： 2120109     DOI： 10.1016/0378-1097(90)90086-6

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  14 in total

1.  The Hha protein as a modulator of expression of virulence factors in Escherichia coli.

Authors:  M Mouriño; C Madrid; C Balsalobre; A Prenafeta; F Muñoa; J Blanco; M Blanco; J E Blanco; A Juarez
Journal:  Infect Immun       Date:  1996-07       Impact factor: 3.441

2.  Cytotoxic necrotizing factor type 1-neutralizing monoclonal antibody NG8 recognizes three amino acids in a C-terminal region of the toxin and reduces toxin binding to HEp-2 cells.

Authors:  Kerian K Grande; Karen C Meysick; Susan B Rasmussen; Alison D O'Brien
Journal:  Infect Immun       Date:  2008-10-27       Impact factor: 3.441

3.  Prevalence and characteristics of Escherichia coli serotype O157:H7 and other verotoxin-producing E. coli in healthy cattle.

Authors:  M Blanco; J E Blanco; J Blanco; E A Gonzalez; A Mora; C Prado; L Fernández; M Rio; J Ramos; M P Alonso
Journal:  Epidemiol Infect       Date:  1996-10       Impact factor: 2.451

4.  Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli.

Authors:  K C Meysick; M Mills; A D O'Brien
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

5.  Production of toxins (enterotoxins, verotoxins, and necrotoxins) and colicins by Escherichia coli strains isolated from septicemic and healthy chickens: relationship with in vivo pathogenicity.

Authors:  J E Blanco; M Blanco; A Mora; J Blanco
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

6.  Cloning, sequencing, and role in virulence of two phospholipases (A1 and C) from mesophilic Aeromonas sp. serogroup O:34.

Authors:  S Merino; A Aguilar; M M Nogueras; M Regue; S Swift; J M Tomás
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

7.  E. coli virulence factors in children with neurogenic bladder associated with bacteriuria.

Authors:  Eliana B M Guidoni; Vanessa A Dalpra; Patrícia Maria Figueiredo; Domingos da Silva Leite; Lycia Mara J Mímica; Tomomasa Yano; Jesus E Blanco; Julio Toporovski
Journal:  Pediatr Nephrol       Date:  2005-12-31       Impact factor: 3.714

8.  Virulence factors and O groups of Escherichia coli isolates from patients with acute pyelonephritis, cystitis and asymptomatic bacteriuria.

Authors:  M Blanco; J E Blanco; M P Alonso; J Blanco
Journal:  Eur J Epidemiol       Date:  1996-04       Impact factor: 8.082

9.  Effect of growth temperature on outer membrane components and virulence of Aeromonas hydrophila strains of serotype O:34.

Authors:  S Merino; S Camprubí; J M Tomás
Journal:  Infect Immun       Date:  1992-10       Impact factor: 3.441

10.  Serotypes of CNF1-producing Escherichia coli strains that cause extraintestinal infections in humans.

Authors:  J E Blanco; J Blanco; M Blanco; M P Alonso; W H Jansen
Journal:  Eur J Epidemiol       Date:  1994-12       Impact factor: 8.082
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