Literature DB >> 2119921

Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests.

C H Carbonetto1, E L Malchiodi, M Chiaramonte, E Durante de Isola, C A Fossati, R A Margni.   

Abstract

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.

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Year:  1990        PMID： 2119921      PMCID： PMC1535144          DOI： 10.1111/j.1365-2249.1990.tb05409.x

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN： 0009-9104            Impact factor:   4.330


  23 in total

1.  Immune precipitation and immunoblotting for the detection of Trypanosoma cruzi antigens.

Authors:  R C Wong; L Hudson; P J Hindmarsh
Journal:  Trans R Soc Trop Med Hyg       Date:  1986       Impact factor: 2.184

2.  Species-specific monoclonal antibodies for a membrane antigen(s) in all developmental forms of Trypanosoma cruzi.

Authors:  H Tachibana; K Nagakura; Y Kaneda
Journal:  Z Parasitenkd       Date:  1986

3.  Trypanosoma cruzi: isolate dependence in the induction of lytic antibodies in the mouse and rabbit.

Authors:  L A Muller; N Añasco; S M González Cappa
Journal:  Exp Parasitol       Date:  1986-06       Impact factor: 2.011

4.  Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody.

Authors:  J L Lemesre; D Afchain; O Orozco; M Loyens; F S Breniere; P Desjeux; Y Carlier; U Martin; J A Nogueira-Queiroz; D Le Ray
Journal:  Am J Trop Med Hyg       Date:  1986-01       Impact factor: 2.345

5.  Analysis of Trypanosoma cruzi antigens bound by specific antibodies and by antibodies to related trypanosomatids.

Authors:  F G Araujo
Journal:  Infect Immun       Date:  1986-07       Impact factor: 3.441

6.  [Immunobiological characterization of a presumed atypical strain of Trypanosoma cruzi].

Authors:  L A Muller; E L de Isola; R D Quintana; S M González Cappa
Journal:  Medicina (B Aires)       Date:  1987       Impact factor: 0.653

7.  Trypanosoma cruzi strain-specific monoclonal antibodies: identification of Colombian strain flagellates in the insect vector.

Authors:  S Giovanni-De-Simone; L C Pontes De Carvalho; O F Oliva; S G Andrade; B Galvao-Castro
Journal:  Trans R Soc Trop Med Hyg       Date:  1987       Impact factor: 2.184

8.  Use of monoclonal antibodies for the differential detection of Trypanosoma cruzi and T. rangeli in epidemiological studies and xenodiagnosis.

Authors:  L Hudson; F Guhl; C J Marinkelle; J Rodriguez
Journal:  Acta Trop       Date:  1987-12       Impact factor: 3.112

9.  A Mr 90 000 surface polypeptide of Trypanosoma cruzi as a candidate for a Chagas' disease diagnostic antigen.

Authors:  E A Dragon; V M Brothers; R A Wrightsman; J Manning
Journal:  Mol Biochem Parasitol       Date:  1985-09       Impact factor: 1.759

Review 10.  Immunoblotting and dot blotting.

Authors:  D I Stott
Journal:  J Immunol Methods       Date:  1989-05-12       Impact factor: 2.303
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  5 in total

1.  Oral vaccination with Salmonella enterica as a cruzipain-DNA delivery system confers protective immunity against Trypanosoma cruzi.

Authors:  Silvia I Cazorla; Pablo D Becker; Fernanda M Frank; Thomas Ebensen; María J Sartori; Ricardo S Corral; Emilio L Malchiodi; Carlos A Guzmán
Journal:  Infect Immun       Date:  2007-10-29       Impact factor: 3.441

2.  Use of full-length recombinant calflagin and its c fragment for improvement of diagnosis of Trypanosoma cruzi infection.

Authors:  Iván S Marcipar; Cintia Roodveldt; Gerardo Corradi; María L Cabeza; Maria Edileuza F Brito; Lucile M Floeter Winter; Alberto J Marcipar; Ariel M Silber
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

3.  Design, construction, and evaluation of a specific chimeric antigen to diagnose chagasic infection.

Authors:  Sebastián Aguirre; Ariel M Silber; Maria Edileuza F Brito; María E Ribone; Claudia M Lagier; Iván S Marcipar
Journal:  J Clin Microbiol       Date:  2006-10       Impact factor: 5.948

4.  Cross-reactivity studies and differential serodiagnosis of human infections caused by Trypanosoma cruzi and Leishmania spp; use of immunoblotting and ELISA with a purified antigen (Ag163B6).

Authors:  E L Malchiodi; M G Chiaramonte; N J Taranto; N W Zwirner; R A Margni
Journal:  Clin Exp Immunol       Date:  1994-09       Impact factor: 4.330

5.  A lytic monoclonal antibody to Trypanosoma cruzi bloodstream trypomastigotes which recognizes an epitope expressed in tissues affected in Chagas' disease.

Authors:  N W Zwirner; E L Malchiodi; M G Chiaramonte; C A Fossati
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441
  5 in total

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