OBJECTIVES: The expression of iNOS mRNA and protein levels was studied in hypothyroid rat cerebral cortex during early days after postnatal life. METHOD: The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis. RESULT: ΔCt in hypothyroid group on day 7, 14, 21 was 18.89±0.92, 18.83±0.99, 16.48±0.29, separately. While in control group, ΔCt was 20.32±0.92, 20.07±0.86, 17.96±0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59 ± 5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37 ± 3%), compared with control group (58 ± 4%). CONCLUSION: Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroid rat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.
OBJECTIVES: The expression of iNOS mRNA and protein levels was studied in hypothyroidrat cerebral cortex during early days after postnatal life. METHOD: The homogenate of the cerebral cortex was examined using Real-time reverse transcription polymerase chain reaction (real-time RT PCR) and Western blot analysis. RESULT: ΔCt in hypothyroid group on day 7, 14, 21 was 18.89±0.92, 18.83±0.99, 16.48±0.29, separately. While in control group, ΔCt was 20.32±0.92, 20.07±0.86, 17.96±0.50, separately. Meanwhile, the protein level was detected only in hypothyroid group of 59 ± 5% on day 14. On day 21, iNOS protein expression showed a decrease (p<0.05) in hypothyroid group (37 ± 3%), compared with control group (58 ± 4%). CONCLUSION: Our findings show the successive changes of the iNOS mRNA and protein levels in early postnatal days of hypothyroidrat brain, and confirm the cross-talk between the TH and NO signaling pathway in developing cerebral cortex of rats.