OBJECTIVE: To compare cardioprotective and anti-inflammatory effects of ischemia preconditioning (IPC) and ischemia postconditioning (IPOC) in a rat myocardial ischemia-reperfusion injury (IRI) model. METHODS: Forty Sprague-Dawley rats were randomly divided equally into four groups. In the groups other than the sham group, the left anterior descending coronary artery was ligated for 30 min followed by a 180 min reperfusion in vivo. The control group was subjected to no additional intervention, the IPC group to three cycles of 5 min ischemia separated by 5 min reperfusion before the index ischemia and the IPOC group to three cycles of 10 s ischemia separated by 10 s reperfusion immediately after the end of the index ischemia. Hemodynamic changes during the ischemia and reperfusion were recorded. At 180 min of reperfusion, serum concentrations of troponin I (TnI), tumor necrosis factor α (TNF-α) and high-mobility group box 1 (HMGB-1) were assayed, and the infarction size was assessed by Evans blue and triphenyltetrazolium chloride staining. RESULTS: Compared to the control group, infarct size and serum concentrations of TnI, TNF-α and HMGB1 at 180 min of reperfusion were significantly reduced in the IPC and IPOC groups. However, infarct size and serum concentrations of TNF-α and HMGB1 at 180 min of reperfusion were significantly increased in the IPOC group compared to the IPC group. CONCLUSIONS: In the rats with myocardial IRI in vivo, both IPC and IPOC can produce significant cardioprotective and anti-inflammatory effects. However, cardioprotective and anti-inflammatory effects provided by IPOC are weaker than with IPC.
OBJECTIVE: To compare cardioprotective and anti-inflammatory effects of ischemia preconditioning (IPC) and ischemia postconditioning (IPOC) in a ratmyocardial ischemia-reperfusion injury (IRI) model. METHODS: Forty Sprague-Dawley rats were randomly divided equally into four groups. In the groups other than the sham group, the left anterior descending coronary artery was ligated for 30 min followed by a 180 min reperfusion in vivo. The control group was subjected to no additional intervention, the IPC group to three cycles of 5 min ischemia separated by 5 min reperfusion before the index ischemia and the IPOC group to three cycles of 10 s ischemia separated by 10 s reperfusion immediately after the end of the index ischemia. Hemodynamic changes during the ischemia and reperfusion were recorded. At 180 min of reperfusion, serum concentrations of troponin I (TnI), tumor necrosis factor α (TNF-α) and high-mobility group box 1 (HMGB-1) were assayed, and the infarction size was assessed by Evans blue and triphenyltetrazolium chloride staining. RESULTS: Compared to the control group, infarct size and serum concentrations of TnI, TNF-α and HMGB1 at 180 min of reperfusion were significantly reduced in the IPC and IPOC groups. However, infarct size and serum concentrations of TNF-α and HMGB1 at 180 min of reperfusion were significantly increased in the IPOC group compared to the IPC group. CONCLUSIONS: In the rats with myocardial IRI in vivo, both IPC and IPOC can produce significant cardioprotective and anti-inflammatory effects. However, cardioprotective and anti-inflammatory effects provided by IPOC are weaker than with IPC.
Authors: T Matsubara; S Minatoguchi; H Matsuo; K Hayakawa; T Segawa; Y Matsuno; S Watanabe; M Arai; Y Uno; M Kawasaki; T Noda; G Takemura; K Nishigaki; H Fujiwara Journal: J Am Coll Cardiol Date: 2000-02 Impact factor: 24.094
Authors: Hajime Kin; Ning-Ping Wang; James Mykytenko; James Reeves; Jeremiah Deneve; Rong Jiang; Amanda J Zatta; Robert A Guyton; Jakob Vinten-Johansen; Zhi-Qing Zhao Journal: Shock Date: 2008-06 Impact factor: 3.454
Authors: Gezina Tanya Mei Ling Oei; Hamid Aslami; Raphaela Priscilla Kerindongo; Renske Johanna Steenstra; Charlotte Jacqueline Peter Beurskens; Anita Maria Tuip-de Boer; Nicole Petra Juffermans; Markus Werner Hollmann; Benedikt Preckel; Nina Claudia Weber Journal: J Immunol Res Date: 2015-01-27 Impact factor: 4.818