Hydrazido-derivatized supports in affinity chromatography.

Many chemistries have been developed for the immobilization of ligands onto insoluble matrices for subsequent use in affinity systems. One such chemistry which has received little attention involves the use of hydrazido-derivatized solid supports. Hydrazine derivatives are strong nucleophiles which will react with a number of functional groups including aldehydes which may be generated on the oligosaccharide moieties of glycoconjugates by specific oxidation reactions. This paper presents a brief overview of the chemistries involved and the uses of hydrazido-derivatized solid supports for the site-directed immobilization of glycoconjugates. Specific examples from the literature on the uses of affinity matrices prepared by this method are cited.