OBJECTIVE: The aim of the present study is to investigate the involvement of immunocompetent cells, pro-inflammatory cytokines and HSP, to evaluate a change of periodontal ligament during the initial stage of orthodontic tooth movement. DESIGN: In the present study, we investigated the distributional density of immunocompetent cells, the localisation of cytokines, and the expression levels of their mRNA in the periodontal ligament during the initial stage of orthodontic tooth movement, using immunohistochemistry and real-time PCR. RESULTS: Orthodontic tooth movement led to significant recruitment of OX6(+) cells and ED1(+) cells in the rat PDL. Double-immunofluorescence staining showed that some ED1(+) cells expressed pro-inflammatory factors of IL-1β and TNF-α in the PDL during orthodontic tooth movement. Real-time PCR analysis revealed that the expression levels of IL-1β (Il1b) and TNF-α (Tnf) mRNA gradually increased following its decrease after 1h of orthodontic tooth movement. These findings suggest that ED1(+) cells are involved in the expression of TNF-α and IL-1β and the subsequent regulation of bone resorption on pressure side. HSP27 (Hspb1) mRNA levels were significantly increased as compared with the control at 1h of the initial stage of treatment. CONCLUSION: ED1(+) cells involved in the expression of TNF-α and IL-1β may play an important role in the initial reaction of the PDL and in the induction of the osteoclastic bone resorption during orthodontic tooth movement.
OBJECTIVE: The aim of the present study is to investigate the involvement of immunocompetent cells, pro-inflammatory cytokines and HSP, to evaluate a change of periodontal ligament during the initial stage of orthodontic tooth movement. DESIGN: In the present study, we investigated the distributional density of immunocompetent cells, the localisation of cytokines, and the expression levels of their mRNA in the periodontal ligament during the initial stage of orthodontic tooth movement, using immunohistochemistry and real-time PCR. RESULTS: Orthodontic tooth movement led to significant recruitment of OX6(+) cells and ED1(+) cells in the rat PDL. Double-immunofluorescence staining showed that some ED1(+) cells expressed pro-inflammatory factors of IL-1β and TNF-α in the PDL during orthodontic tooth movement. Real-time PCR analysis revealed that the expression levels of IL-1β (Il1b) and TNF-α (Tnf) mRNA gradually increased following its decrease after 1h of orthodontic tooth movement. These findings suggest that ED1(+) cells are involved in the expression of TNF-α and IL-1β and the subsequent regulation of bone resorption on pressure side. HSP27 (Hspb1) mRNA levels were significantly increased as compared with the control at 1h of the initial stage of treatment. CONCLUSION:ED1(+) cells involved in the expression of TNF-α and IL-1β may play an important role in the initial reaction of the PDL and in the induction of the osteoclastic bone resorption during orthodontic tooth movement.
Authors: M Di Domenico; F D'apuzzo; A Feola; L Cito; A Monsurrò; G M Pierantoni; L Berrino; A De Rosa; A Polimeni; L Perillo Journal: J Biomed Biotechnol Date: 2012-05-14