Literature DB >> 21191053

Rapid detection of Mycobacterium tuberculosis complex and rifampin resistance in smear-negative clinical samples by use of an integrated real-time PCR method.

Raquel Moure1, Laura Muñoz, Miriam Torres, Miguel Santin, Rogelio Martín, Fernando Alcaide.   

Abstract

Sixty-four of 85 (75.3%) smear-negative respiratory (n = 78) and nonrespiratory (n = 7) samples with positive cultures of Mycobacterium tuberculosis complex (MTC) were detected by the GeneXpert system using the Xpert MTB/RIF assay (GX). In addition, GX found rpoB mutations in all six of the rifampin-resistant strains detected. The test was negative in 20 culture-negative and 20 nontuberculous culture-positive samples (100% specificity). GX offers high potential for the diagnosis of tuberculosis due to its capacity for direct detection of MTC, its rapidity, and its simplicity.

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Year:  2010        PMID： 21191053      PMCID： PMC3067676          DOI： 10.1128/JCM.01831-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Comparison of the real-time PCR method and the Gen-Probe amplified Mycobacterium tuberculosis direct test for detection of Mycobacterium tuberculosis in pulmonary and nonpulmonary specimens.

Authors:  Nadine Lemaître; Sylvie Armand; Anne Vachée; Odile Capilliez; Christine Dumoulin; René J Courcol
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

2.  Rapid diagnosis of pulmonary tuberculosis using Roche AMPLICOR Mycobacterium tuberculosis PCR test.

Authors:  R F D'Amato; A Miller
Journal:  Methods Mol Biol       Date:  1998

3.  Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis.

Authors:  A S Piatek; S Tyagi; A C Pol; A Telenti; L P Miller; F R Kramer; D Alland
Journal:  Nat Biotechnol       Date:  1998-04       Impact factor: 54.908

4.  Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis.

Authors:  A Telenti; P Imboden; F Marchesi; D Lowrie; S Cole; M J Colston; L Matter; K Schopfer; T Bodmer
Journal:  Lancet       Date:  1993-03-13       Impact factor: 79.321

5.  Application of the Genotype MTBDR assay directly on sputum specimens.

Authors:  D Hillemann; S Rüsch-Gerdes; E Richter
Journal:  Int J Tuberc Lung Dis       Date:  2006-09       Impact factor: 2.373

Review 6.  A systematic review of rapid diagnostic tests for the detection of tuberculosis infection.

Authors:  J Dinnes; J Deeks; H Kunst; A Gibson; E Cummins; N Waugh; F Drobniewski; A Lalvani
Journal:  Health Technol Assess       Date:  2007-01       Impact factor: 4.014

7.  Reduction in turnaround time for laboratory diagnosis of pulmonary tuberculosis by routine use of a nucleic acid amplification test.

Authors:  Douglas F Moore; Joseph A Guzman; Lydia T Mikhail
Journal:  Diagn Microbiol Infect Dis       Date:  2005-07       Impact factor: 2.803

8.  Transmission of Mycobacterium tuberculosis from patients smear-negative for acid-fast bacilli.

Authors:  M A Behr; S A Warren; H Salamon; P C Hopewell; A Ponce de Leon; C L Daley; P M Small
Journal:  Lancet       Date:  1999-02-06       Impact factor: 79.321

9.  The clinical impact of nucleic acid amplification tests on the diagnosis and management of tuberculosis in a British hospital.

Authors:  M Taegtmeyer; N J Beeching; J Scott; K Seddon; S Jamieson; S B Squire; H C Mwandumba; A R O Miller; P D O Davies; C M Parry
Journal:  Thorax       Date:  2007-11-16       Impact factor: 9.139

10.  Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex.

Authors:  C E Musial; L S Tice; L Stockman; G D Roberts
Journal:  J Clin Microbiol       Date:  1988-10       Impact factor: 5.948
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  74 in total

1.  Effectiveness of an integrated real-time PCR method for detection of the Mycobacterium tuberculosis complex in smear-negative extrapulmonary samples in an area of low tuberculosis prevalence.

Authors:  Raquel Moure; Rogelio Martín; Fernando Alcaide
Journal:  J Clin Microbiol       Date:  2011-12-07       Impact factor: 5.948

2.  Direct detection of Mycobacterium tuberculosis complex in clinical samples by a molecular method based on GenoQuick technology.

Authors:  Raquel Moure; Miriam Torres; Rogelio Martín; Fernando Alcaide
Journal:  J Clin Microbiol       Date:  2012-03-14       Impact factor: 5.948

3.  Silent mutation in rpoB detected from clinical samples with rifampin-susceptible Mycobacterium tuberculosis.

Authors:  Raquel Moure; Rogelio Martín; Fernando Alcaide
Journal:  J Clin Microbiol       Date:  2011-10       Impact factor: 5.948

4.  Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

Authors:  L E Scott; N Gous; B E Cunningham; B D Kana; O Perovic; L Erasmus; G J Coetzee; H Koornhof; W Stevens
Journal:  J Clin Microbiol       Date:  2011-10-05       Impact factor: 5.948

5.  Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens.

Authors:  Arzu N Zeka; Sezai Tasbakan; Cengiz Cavusoglu
Journal:  J Clin Microbiol       Date:  2011-09-28       Impact factor: 5.948

Review 6.  Xpert® MTB/RIF assay: development, evaluation and implementation of a new rapid molecular diagnostic for tuberculosis and rifampicin resistance.

Authors:  Stephen D Lawn; Mark P Nicol
Journal:  Future Microbiol       Date:  2011-09       Impact factor: 3.165

7.  A multisite assessment of the quantitative capabilities of the Xpert MTB/RIF assay.

Authors:  Robert Blakemore; Pamela Nabeta; Amy L Davidow; Viral Vadwai; Rasim Tahirli; Vanisha Munsamy; Mark Nicol; Martin Jones; David H Persing; Doris Hillemann; Sabine Ruesch-Gerdes; Felicity Leisegang; Carlos Zamudio; Camilla Rodrigues; Catharina C Boehme; Mark D Perkins; David Alland
Journal:  Am J Respir Crit Care Med       Date:  2011-11-01       Impact factor: 21.405

8.  Cepheid GeneXpert MTB/RIF assay for Mycobacterium tuberculosis detection and rifampin resistance identification in patients with substantial clinical indications of tuberculosis and smear-negative microscopy results.

Authors:  Panayotis Ioannidis; Dimitrios Papaventsis; Simona Karabela; Stavroula Nikolaou; Marina Panagi; Ekaterini Raftopoulou; Eythymia Konstantinidou; Ioanna Marinou; Sofia Kanavaki
Journal:  J Clin Microbiol       Date:  2011-06-15       Impact factor: 5.948

9.  Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens.

Authors:  Sylvie Armand; Pascale Vanhuls; Guy Delcroix; René Courcol; Nadine Lemaître
Journal:  J Clin Microbiol       Date:  2011-03-16       Impact factor: 5.948

Review 10.  Xpert® MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance.

Authors:  Mikashmi Kohli; Ian Schiller; Nandini Dendukuri; Keertan Dheda; Claudia M Denkinger; Samuel G Schumacher; Karen R Steingart
Journal:  Cochrane Database Syst Rev       Date:  2018-08-27
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