Literature DB >> 21185800

Kinetic and stoichiometric analysis of the modification process for N-terminal PEGylation of staphylokinase.

Jun Wang1, Tao Hu, Yongdong Liu, Guifeng Zhang, Guanghui Ma, Zhiguo Su.   

Abstract

Staphylokinase (SAK) is a therapeutic protein with promise for thrombolytic therapy of acute myocardial infarction. In this study, polyethylene glycol (PEG) aldehyde was used for N-terminal PEGylation of SAK to improve the pharmacological profiles of SAK. Due to the presence of the competitive PEGylation between the N terminus and the Lys residues, kinetic and stoichiometric analysis was carried out to investigate the process for the N-terminal PEGylation of SAK. To achieve this objective, size exclusion chromatography and tryptic peptide mapping were used to measure the PEGylation extent of SAK molecule and its specific amino acid residues, respectively.
Copyright © 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 21185800     DOI： 10.1016/j.ab.2010.12.030

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  N-terminal protein modification using simple aminoacyl transferase substrates.

Authors:  Anne M Wagner; Mark W Fegley; John B Warner; Christina L J Grindley; Nicholas P Marotta; E James Petersson
Journal:  J Am Chem Soc       Date:  2011-09-06       Impact factor: 15.419

2.  Expression, Purification, and Polyethylene Glycol Site-Specific Modification of Recombinant Human Interleukin 24 in Escherichia coli.

Authors:  Yao Zhang; Qunfeng Ma; Junfeng Wang; Jianlin Ge; Jilei Hua; Yinan Shi; Chi Zhang; Mengzhe Liu; Yuqi Wang; Zhinan Chen; Ziling Wang; Yongdong Liu; Hong Jiang
Journal:  Protein J       Date:  2019-10       Impact factor: 2.371

3.  Impact of the PEG length and PEGylation site on the structural, thermodynamic, thermal, and proteolytic stability of mono-PEGylated alpha-1 antitrypsin.

Authors:  Xiao Liu; Kobenan G W Kouassi; Rita Vanbever; Mireille Dumoulin
Journal:  Protein Sci       Date:  2022-09       Impact factor: 6.993

4.  Bioactivity and circulation time of PEGylated NELL-1 in mice and the potential for osteoporosis therapy.

Authors:  Yulong Zhang; Omar Velasco; Xinli Zhang; Kang Ting; Chia Soo; Benjamin M Wu
Journal:  Biomaterials       Date:  2014-05-10       Impact factor: 12.479

5.  The Positive Correlation of the Enhanced Immune Response to PCV2 Subunit Vaccine by Conjugation of Chitosan Oligosaccharide with the Deacetylation Degree.

Authors:  Guiqiang Zhang; Gong Cheng; Peiyuan Jia; Siming Jiao; Cui Feng; Tao Hu; Hongtao Liu; Yuguang Du
Journal:  Mar Drugs       Date:  2017-07-26       Impact factor: 5.118

6.  Molecular insight into the steric shielding effect of PEG on the conjugated staphylokinase: biochemical characterization and molecular dynamics simulation.

Authors:  Qimeng Mu; Tao Hu; Jingkai Yu
Journal:  PLoS One       Date:  2013-07-18       Impact factor: 3.240
  6 in total

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