Literature DB >> 21185331

Increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time RT-PCR assay.

Xiaoli Pang1, Min Cao, Min Zhang, Bonita Lee.   

Abstract

The real-time TaqMan RT-PCR assay (Pang et al., 2004) did not detect 14 clinical samples with rotavirus G2 genotype. Three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to G2P[4], G2P[8], G3P[4], G9P[4], G8 and G12 sequences. An additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus G and P genotypes. The modified assay has improved significantly the sensitivity for specific rotavirus strains without affecting the detection of other genotypes, creating a molecular assay with broad detection of various genotypes of group A rotaviruses.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 21185331     DOI： 10.1016/j.jviromet.2010.12.013

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  16 in total

1.  A Somatic Coliphage Threshold Approach To Improve the Management of Activated Sludge Wastewater Treatment Plant Effluents in Resource-Limited Regions.

Authors:  Luz Chacón; Kenia Barrantes; Carolina Santamaría-Ulloa; Melissa Solano; Liliana Reyes; Lizeth Taylor; Carmen Valiente; Erin M Symonds; Rosario Achí
Journal:  Appl Environ Microbiol       Date:  2020-08-18       Impact factor: 4.792

2.  Impact of xynthia tempest on viral contamination of shellfish.

Authors:  Marco Grodzki; Joanna Ollivier; Jean-Claude Le Saux; Jean-Côme Piquet; Mathilde Noyer; Françoise S Le Guyader
Journal:  Appl Environ Microbiol       Date:  2012-02-17       Impact factor: 4.792

3.  Detection of Norovirus and Rotavirus Present in Suspended and Dissolved Forms in Drinking Water Sources.

Authors:  Takayuki Miura; Arisa Gima; Michihiro Akiba
Journal:  Food Environ Virol       Date:  2018-12-17       Impact factor: 2.778

4.  Rotavirus shedding following administration of RV3-BB human neonatal rotavirus vaccine.

Authors:  Daniel Cowley; Karen Boniface; Nada Bogdanovic-Sakran; Carl D Kirkwood; Julie E Bines
Journal:  Hum Vaccin Immunother       Date:  2017-05-08       Impact factor: 3.452

5.  Environmental detection of genogroup I, II, and IV noroviruses by using a generic real-time reverse transcription-PCR assay.

Authors:  Takayuki Miura; Sylvain Parnaudeau; Marco Grodzki; Satoshi Okabe; Robert L Atmar; Françoise S Le Guyader
Journal:  Appl Environ Microbiol       Date:  2013-08-16       Impact factor: 4.792

6.  Sensitive and specific quantitative detection of rotavirus A by one-step real-time reverse transcription-PCR assay without antecedent double-stranded-RNA denaturation.

Authors:  Slavica Mijatovic-Rustempasic; Ka Ian Tam; Tara K Kerin; Jamie M Lewis; Rashi Gautam; Osbourne Quaye; Jon R Gentsch; Michael D Bowen
Journal:  J Clin Microbiol       Date:  2013-07-12       Impact factor: 5.948

7.  Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process.

Authors:  Takayuki Miura; Julien Schaeffer; Jean-Claude Le Saux; Philippe Le Mehaute; Françoise S Le Guyader
Journal:  Food Environ Virol       Date:  2017-12-06       Impact factor: 2.778

8.  Rapid genotyping of human rotavirus using SYBR green real-time reverse transcription-polymerase chain reaction with melting curve analysis.

Authors:  Yupin Tong; Bonita E Lee; Xiaoli L Pang
Journal:  World J Virol       Date:  2015-11-12

9.  Recovery of Nucleic Acids of Enteric Viruses and Host-Specific Bacteroidales from Groundwater by Using an Adsorption-Direct Extraction Method.

Authors:  Takayuki Miura; Hiroyuki Takino; Arisa Gima; Eiji Haramoto; Michihiro Akiba
Journal:  Appl Environ Microbiol       Date:  2021-08-26       Impact factor: 4.792

10.  Observational Research in Childhood Infectious Diseases (ORChID): a dynamic birth cohort study.

Authors:  Stephen Bernard Lambert; Robert S Ware; Anne L Cook; Frances A Maguire; David M Whiley; Seweryn Bialasiewicz; Ian M Mackay; David Wang; Theo P Sloots; Michael D Nissen; Keith Grimwood
Journal:  BMJ Open       Date:  2012-10-31       Impact factor: 2.692
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