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Literature DB >> 2118341

The effects of pH and temperature on fluorescent calcium indicators as determined with Chelex-100 and EDTA buffer systems.

Abstract

A novel method of determining the apparent dissociation constants of fluorescent calcium indicators is described which utilizes Chelex-100 ion exchange resin and 45Ca. The affinity for calcium of indicators fluo-3, fura-2 and indo-1 measured at either 22 degrees or 37 degrees C decreases as pH is decreased from 7.4 to 5.5. These measurements agree with determinations made using EDTA-calcium buffers. The 1:1 calcium:indicator complex is maintained under all conditions. The necessity to correct dissociation constants during intracellular acidification to properly interpret fluorescence measurements is illustrated by indo-1 measurements in the ischemic rat heart.

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Year: 1990 PMID： 2118341 DOI： 10.1016/0006-291x(90)91362-v

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

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Cited

36 in total

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Authors: T Nakamura; H Hayashi; H Satoh; H Katoh; M Kaneko; H Terada
Journal: Mol Cell Biochem Date: 1999-04 Impact factor: 3.396

2. Effects of temperature on calcium-sensitive fluorescent probes.

Authors: A E Oliver; G A Baker; R D Fugate; F Tablin; J H Crowe
Journal: Biophys J Date: 2000-04 Impact factor: 4.033

3. The relationship between the action potential, intracellular calcium and force in intact phasic, guinea-pig uretic smooth muscle.

Authors: T V Burdyga; S Wray
Journal: J Physiol Date: 1999-11-01 Impact factor: 5.182

4. TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells.

Authors: Nandhini Muthukrishnan; Gregory A Johnson; Jongdoo Lim; Eric E Simanek; Jean-Philippe Pellois
Journal: Biochim Biophys Acta Date: 2012-07-03

The effects of pH and temperature on fluorescent calcium indicators as determined with Chelex-100 and EDTA buffer systems.

1. A single cell model of myocardial reperfusion injury: changes in intracellular Na+ and Ca2+ concentrations in guinea pig ventricular myocytes.

2. Effects of temperature on calcium-sensitive fluorescent probes.

3. The relationship between the action potential, intracellular calcium and force in intact phasic, guinea-pig uretic smooth muscle.

4. TAT-mediated photochemical internalization results in cell killing by causing the release of calcium into the cytosol of cells.

5. Two phases of zymogen granule lifetime in mouse pancreas: ghost granules linger after exocytosis of contents.

6. Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.

7. Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes.

Review 8. Mechanisms of acute ischemic contractile failure of the heart. Role of intracellular calcium.

9. Stimulus-dependent modulation of smooth muscle intracellular calcium and force by altered intracellular pH.

10. Menthol response and adaptation in nociceptive-like and nonnociceptive-like neurons: role of protein kinases.