| Literature DB >> 21181525 |
Akio Kato1, Jonathan C Lamb, Patrice S Albert, Tatiana Danilova, Fangpu Han, Zhi Gao, Seth Findley, James A Birchler.
Abstract
Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress. This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on somatic metaphase and meiotic pachytene spreads.Mesh：
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Year: 2011 PMID： 21181525 DOI： 10.1007/978-1-61737-957-4_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745