INTRODUCTION: Candidiasis is one of the most common fungal infections among patients infected by human immunodeficiency virus. The present study aimed to characterize yeasts of the genus Candida from distinct clinical samples from HIV-positive patients and determine the in vitro susceptibility profile to five antifungal drugs. METHODS: Characterization of Candida sp was achieved using the classic methodology: biochemical (zymogram and auxanogram) and micromorphology (germinative tube growth test and slide microculture) tests. Genotypic technique (PCR) and identification by the commercial method API 20C AUX (Biomeriéux) were also performed. To determine the in vitro susceptibility profile, five antifungal drugs were used (ketoconazole, fluconazole, itraconazole, voriconazole and amphotericin-B) following a commercially available method, the Etest. RESULTS: The procedure isolated 105 yeasts of the genus Candida from 102 HIV-infected patients. Of these, 82 (78.1%) were characterized as Candida albicans, 8 (7.6%) as C. parapsilosi s, 8 (7.6%) C. tropicalis, 4 (3.8%) C. krusei, 2 (1.9%) C. glabrata, and 1 (1%) as C. guiilliermondii. CONCLUSIONS: Considering the general profile of sensitivity, 60% of isolates were susceptible to all the antifungal drugs tested; however, the species C. tropicalis and C. krusei showed a tendency toward higher MICs to azoles than those obtained for C. albicans, suggesting resistance.
INTRODUCTION:Candidiasis is one of the most common fungal infections among patients infected by human immunodeficiency virus. The present study aimed to characterize yeasts of the genus Candida from distinct clinical samples from HIV-positivepatients and determine the in vitro susceptibility profile to five antifungal drugs. METHODS: Characterization of Candida sp was achieved using the classic methodology: biochemical (zymogram and auxanogram) and micromorphology (germinative tube growth test and slide microculture) tests. Genotypic technique (PCR) and identification by the commercial method API 20C AUX (Biomeriéux) were also performed. To determine the in vitro susceptibility profile, five antifungal drugs were used (ketoconazole, fluconazole, itraconazole, voriconazole and amphotericin-B) following a commercially available method, the Etest. RESULTS: The procedure isolated 105 yeasts of the genus Candida from 102 HIV-infectedpatients. Of these, 82 (78.1%) were characterized as Candida albicans, 8 (7.6%) as C. parapsilosi s, 8 (7.6%) C. tropicalis, 4 (3.8%) C. krusei, 2 (1.9%) C. glabrata, and 1 (1%) as C. guiilliermondii. CONCLUSIONS: Considering the general profile of sensitivity, 60% of isolates were susceptible to all the antifungal drugs tested; however, the species C. tropicalis and C. krusei showed a tendency toward higher MICs to azoles than those obtained for C. albicans, suggesting resistance.
Authors: Taís Gusmão da Silva; Josefa Carolaine Pereira da Silva; Joara Nályda Pereira Carneiro; Wanderlei do Amaral; Cícero Deschamps; Jenifer Priscila de Araújo; José Galberto Martins da Costa; Waltécio de Oliveira Almeida; Luiz Everson da Silva; Henrique Douglas Melo Coutinho; Jaime Ribeiro Filho; Maria Flaviana Bezerra Morais-Braga Journal: Arch Microbiol Date: 2021-03-31 Impact factor: 2.552
Authors: Natália Spillere Rovaris; Dayani Galato; Fabiana Schuelter-Trevisol; Jane da Silva; Leandro da Silva Linhar; Daniela Alba Nickel; Jefferson Traebert Journal: BMC Res Notes Date: 2014-07-28
Authors: Analucia G Terças; Andrea de Souza Monteiro; Eduardo B Moffa; Julliana R A Dos Santos; Eduardo M de Sousa; Anna R B Pinto; Paola C da Silva Costa; Antonio C R Borges; Luce M B Torres; Allan K D Barros Filho; Elizabeth S Fernandes; Cristina de Andrade Monteiro Journal: Front Microbiol Date: 2017-04-10 Impact factor: 5.640