| Literature DB >> 21176865 |
Erica S Martins-Duarte1, Leandro Lemgruber, Silvia Orenes Lorente, Ludovic Gros, Filippo Magaraci, Ian H Gilbert, Wanderley de Souza, Rossiane C Vommaro.
Abstract
Previous studies from our group have demonstrated the high susceptibility of Toxoplasma gondii tachyzoites to the sterol analogues 22,26-azasterol and 24,25-(R,S)-epiminolanosterol. In this work we present data on testing in vitro three novel azasterols as potential agents for the treatment of toxoplasmosis. The three compounds inhibited parasite growth at micromolar concentrations, in a dose-dependent manner. Electron microscopy analysis of intracellular tachyzoites after treatment with the most effective compound showed drastic mitochondrion swelling associated with the appearance of an electron-lucent matrix and disrupted cristae. Parasite lysis also took place. The appearance of electron dense cytoplasmic structures similar to amylopectin granules distributed throughout the parasite suggests that azasterols might be inducing differentiation of those tachyzoites which were not lysed to the bradyzoite stage.Entities:
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Year: 2010 PMID： 21176865 DOI： 10.1016/j.vetpar.2010.11.034
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738