OBJECTIVE: This study evaluated the effects of photodynamic therapy on pathogenicity of Candida albicans. STUDY DESIGN: Fifty-six rats were submitted to development of candidiasis on the tongue dorsum by C. albicans inoculations. After 5 days, different treatments were administered: laser and photosynthesizer methylene blue (L+P+); laser only (L+P-); photosensitizer only (L-P+); and physiologic solution only (L-P-). Samples of the oral cavity were collected for a count of colony-forming units per mL. Colonies were isolated for evaluation of proteinase and phospholipase activities. The rats were killed for microscopic analysis of the tongue dorsum. The data were analyzed by analysis of variance, Kruskal-Wallis, and Bonferroni tests. RESULTS: The number of C. albicans recovered from the oral cavity of the rats was similar between the groups (P = .106). The L+P+ group showed fewer microscopic lesions of candidiasis than the L-P- group (P = .001). The L+P+ group presented lower proteinase activity compared with the other groups, with significant difference between the groups L+P+ and L-P+ (P = .018). CONCLUSIONS: Photodynamic therapy reduced the microscopic lesions of experimental candidiasis in rats and inhibited the proteinase activity of C. albicans.
OBJECTIVE: This study evaluated the effects of photodynamic therapy on pathogenicity of Candida albicans. STUDY DESIGN: Fifty-six rats were submitted to development of candidiasis on the tongue dorsum by C. albicans inoculations. After 5 days, different treatments were administered: laser and photosynthesizer methylene blue (L+P+); laser only (L+P-); photosensitizer only (L-P+); and physiologic solution only (L-P-). Samples of the oral cavity were collected for a count of colony-forming units per mL. Colonies were isolated for evaluation of proteinase and phospholipase activities. The rats were killed for microscopic analysis of the tongue dorsum. The data were analyzed by analysis of variance, Kruskal-Wallis, and Bonferroni tests. RESULTS: The number of C. albicans recovered from the oral cavity of the rats was similar between the groups (P = .106). The L+P+ group showed fewer microscopic lesions of candidiasis than the L-P- group (P = .001). The L+P+ group presented lower proteinase activity compared with the other groups, with significant difference between the groups L+P+ and L-P+ (P = .018). CONCLUSIONS: Photodynamic therapy reduced the microscopic lesions of experimental candidiasis in rats and inhibited the proteinase activity of C. albicans.
Authors: Nathalia Ramos da Silva; Daniela Garcia Ribeiro; João Paulo Mardegan Issa; Karla Bonfá; Michelli Sobreiro Menezes; Viviane de Cássia Oliveira; Raphael Freitas de Souza Journal: Lasers Med Sci Date: 2017-05-15 Impact factor: 3.161
Authors: Fernanda Freire; Cleber Ferraresi; Antonio Olavo C Jorge; Michael R Hamblin Journal: J Photochem Photobiol B Date: 2016-04-01 Impact factor: 6.252
Authors: Aleksandra Taraszkiewicz; Grzegorz Szewczyk; Tadeusz Sarna; Krzysztof P Bielawski; Joanna Nakonieczna Journal: PLoS One Date: 2015-06-08 Impact factor: 3.240
Authors: Mario Pérez-Sayáns; Renee Beiro-Fuentes; Eva M Otero-Rey; Cintia Micaela Chamorro-Petronacci; Pilar Gándara-Vila; Jose Manuel Somoza-Martín; Abel García-García; Andrés Blanco-Carrión Journal: J Dent Sci Date: 2020-07-12 Impact factor: 2.080
Authors: José Chibebe Junior; Beth B Fuchs; Caetano P Sabino; Juliana C Junqueira; Antonio O C Jorge; Martha S Ribeiro; Michael S Gilmore; Louis B Rice; George P Tegos; Michael R Hamblin; Eleftherios Mylonakis Journal: PLoS One Date: 2013-02-14 Impact factor: 3.240