Literature DB >> 2117559

Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met).

J Bratty1, T F Wu, K Nicoghosian, K K Ogilvie, J P Perreault, G Keith, R Cedergren.   

Abstract

A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2117559     DOI： 10.1016/0014-5793(90)81118-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

1.  Structural requirements for enzymatic formation of threonylcarbamoyladenosine (t6A) in tRNA: an in vivo study with Xenopus laevis oocytes.

Authors:  A Morin; S Auxilien; B Senger; R Tewari; H Grosjean
Journal:  RNA       Date:  1998-01       Impact factor: 4.942

2.  Oligoribonucleotides containing 2',5'-phosphodiester linkages exhibit binding selectivity for 3',5'-RNA over 3',5'-ssDNA.

Authors:  P A Giannaris; M J Damha
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

3.  Preparation of biologically active Ascaris suum mitochondrial tRNAMet with a TV-replacement loop by ligation of chemically synthesized RNA fragments.

Authors:  T Ohtsuki; G Kawai; Y Watanabe; K Kita; K Nishikawa; K Watanabe
Journal:  Nucleic Acids Res       Date:  1996-02-15       Impact factor: 16.971

4.  Chemical synthesis of a very long oligoribonucleotide with 2-cyanoethoxymethyl (CEM) as the 2'-O-protecting group: structural identification and biological activity of a synthetic 110mer precursor-microRNA candidate.

Authors:  Yoshinobu Shiba; Hirofumi Masuda; Naoki Watanabe; Takeshi Ego; Kazuchika Takagaki; Kouichi Ishiyama; Tadaaki Ohgi; Junichi Yano
Journal:  Nucleic Acids Res       Date:  2007-04-25       Impact factor: 16.971
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.