Literature DB >> 2117459

16S rRNA sequences reveal uncultured inhabitants of a well-studied thermal community.

D M Ward1, R Weller, M M Bateson.   

Abstract

Molecular methods are beginning to reveal inhabitants of natural microbial communities which have never before been cultured. Our approach involves selective cloning of naturally occurring 16S rRNA sequences as cDNA, and comparison of these sequences to a database which includes 16S rRNA sequences of isolated community members. We provide here an overview of the method and its potential for community analysis. A 16S rRNA sequence retrieved from the well-studied hot spring cyanobacterial mat in Octopus Spring (Yellowstone National Park) is shown as an example of one contributed by an uncultured member of the community.

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Year:  1990        PMID: 2117459     DOI: 10.1111/j.1574-6968.1990.tb04088.x

Source DB:  PubMed          Journal:  FEMS Microbiol Rev        ISSN: 0168-6445            Impact factor:   16.408


  30 in total

1.  Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes.

Authors:  H Dang; C R Lovell
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Enrichment culture and microscopy conceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat.

Authors:  M J Ferris; A L Ruff-Roberts; E D Kopczynski; M M Bateson; D M Ward
Journal:  Appl Environ Microbiol       Date:  1996-03       Impact factor: 4.792

3.  Wide distribution and diversity of members of the bacterial kingdom Acidobacterium in the environment.

Authors:  S M Barns; S L Takala; C R Kuske
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

4.  Phototrophic phylotypes dominate mesothermal microbial mats associated with hot springs in Yellowstone National Park.

Authors:  Kimberly A Ross; Leah M Feazel; Charles E Robertson; Babu Z Fathepure; Katherine E Wright; Rebecca M Turk-Macleod; Mallory M Chan; Nicole L Held; John R Spear; Norman R Pace
Journal:  Microb Ecol       Date:  2012-02-11       Impact factor: 4.552

5.  Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients.

Authors:  H Heuer; M Krsek; P Baker; K Smalla; E M Wellington
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

6.  16S rRNA sequences of uncultivated hot spring cyanobacterial mat inhabitants retrieved as randomly primed cDNA.

Authors:  R Weller; J W Weller; D M Ward
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

7.  Differential microbial communities in hot spring mats from Western Thailand.

Authors:  M C Portillo; V Sririn; W Kanoksilapatham; J M Gonzalez
Journal:  Extremophiles       Date:  2008-12-25       Impact factor: 2.395

8.  Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis.

Authors:  M J Ferris; D M Ward
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792

Review 9.  A natural view of microbial biodiversity within hot spring cyanobacterial mat communities.

Authors:  D M Ward; M J Ferris; S C Nold; M M Bateson
Journal:  Microbiol Mol Biol Rev       Date:  1998-12       Impact factor: 11.056

10.  Phylogenetic analysis of the bacterial communities in marine sediments.

Authors:  J P Gray; R P Herwig
Journal:  Appl Environ Microbiol       Date:  1996-11       Impact factor: 4.792

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