Literature DB >> 21167881

Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis.

Aurélie Motré1, Richard Kong, Ying Li.   

Abstract

In this study, we report the development of a helicase-dependent amplification assay for rapid detection of Mycobacterium tuberculosis (MTB). By applying a step-by-step optimization method, the amplification time from an input of 2-copy MTB genomic DNA was reduced from about 60 min to less than 30 min.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 21167881     DOI: 10.1016/j.mimet.2010.12.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  6 in total

1.  Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae detection in urine, endocervical, and vaginal specimens by a multiplexed isothermal thermophilic helicase-dependent amplification (tHDA) assay.

Authors:  Dominic O'Neil; Victoria Doseeva; Thomas Rothmann; John Wolff; Irina Nazarenko
Journal:  J Clin Microbiol       Date:  2011-09-28       Impact factor: 5.948

Review 2.  Alternative molecular tests for virological diagnosis.

Authors:  Francesca Sidoti; Massimiliano Bergallo; Cristina Costa; Rossana Cavallo
Journal:  Mol Biotechnol       Date:  2013-03       Impact factor: 2.695

Review 3.  Diagnostic devices for isothermal nucleic acid amplification.

Authors:  Chia-Chen Chang; Chien-Cheng Chen; Shih-Chung Wei; Hui-Hsin Lu; Yang-Hung Liang; Chii-Wann Lin
Journal:  Sensors (Basel)       Date:  2012-06-14       Impact factor: 3.576

4.  Low cost extraction and isothermal amplification of DNA for infectious diarrhea diagnosis.

Authors:  Shichu Huang; Jaephil Do; Madhumita Mahalanabis; Andy Fan; Lei Zhao; Lisa Jepeal; Satish K Singh; Catherine M Klapperich
Journal:  PLoS One       Date:  2013-03-28       Impact factor: 3.240

5.  Evaluation of the specificity and sensitivity of a potential rapid influenza screening system.

Authors:  Luis E Perez; Gerald A Merrill; Robert A DeLorenzo; Thomas W Schoenfeld; Abhay Vats; Michael J Moser
Journal:  Diagn Microbiol Infect Dis       Date:  2012-10-18       Impact factor: 2.803

6.  An isothermal amplification-based point-of-care diagnostic platform for the detection of Mycobacterium tuberculosis: A proof-of-concept study.

Authors:  Rathina Kumar Shanmugakani; Wesley Bonam; David Erickson; Saurabh Mehta
Journal:  Curr Res Biotechnol       Date:  2021-05-21
  6 in total

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