Literature DB >> 21165442

The use of immunohistochemical method for detection of brain microglia in paraffin sections.

E G Suchorukova1, O V Kirik, D E Korzhevskii.   

Abstract

We developed a well reproducible method for detection of brain microglia on paraffin sections on the basis of modern immunohistochemical methods. Polyclonal antibodies to Iba1 antigen obtained after immunization with a synthetic peptide corresponding to 81-93 amino acid fragment of Iba1 antigen selectively interact with microglia cells on paraffin sections of rat brain. Detailed protocol of preparation treatment was drawn up ensuring complete detection of the microglia expressing Iba1 protein.

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Year:  2010        PMID： 21165442     DOI： 10.1007/s10517-010-1048-3

Source DB:  PubMed          Journal:  Bull Exp Biol Med        ISSN： 0007-4888            Impact factor:   0.804


  4 in total

1.  Relaxin increases sympathetic nerve activity and activates spinally projecting neurons in the paraventricular nucleus of nonpregnant, but not pregnant, rats.

Authors:  K Max Coldren; Randall Brown; Eileen M Hasser; Cheryl M Heesch
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2015-09-23       Impact factor: 3.619

2.  Glial reaction in the spinal cord of the degenerating muscle mouse (Scn8a (dmu)).

Authors:  Tadasu Sato; Masatoshi Fujita; Yoshinaka Shimizu; Hiroyasu Kanetaka; Leona W G Chu; Patrice D Côté; Hiroyuki Ichikawa
Journal:  Neurochem Res       Date:  2014-11-08       Impact factor: 3.996

3.  Prednisolone induces microglial activation in the subnucleus caudalis of the rat trigeminal sensory complex.

Authors:  Yu Endo; Noriaki Shoji; Yusuke Shimada; Eriko Kasahara; Masahiro Iikubo; Tadasu Sato; Takashi Sasano; Hiroyuki Ichikawa
Journal:  Cell Mol Neurobiol       Date:  2014-01       Impact factor: 5.046

4.  Immunohistochemical demonstration of specific antigens in the human brain fixed in zinc-ethanol-formaldehyde.

Authors:  D E Korzhevskii; E G Sukhorukova; O V Kirik; I P Grigorev
Journal:  Eur J Histochem       Date:  2015-08-05       Impact factor: 3.188
  4 in total

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