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Literature DB >> 2116452

Buffered charcoal-yeast extract medium for the isolation of brucellae.

Abstract

A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar. Sixteen strains of various Brucella species were inoculated on BCYE agar; selective BCYE agar with polymyxin, anisomycin, and cefamandole; and brucella blood agar. The growth and recovery rates on the three media tested were comparable for 14 strains. BCYE agar with polymyxin, anisomycin, and cefamandole may be useful as a selective medium for the isolation of brucellae.

Entities: Chemical Disease Species

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Year: 1990 PMID： 2116452 PMCID： PMC268015 DOI： 10.1128/jcm.28.7.1671-1672.1990

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. Brucellosis of the bones and joints. Experience with thirty-six patients.

Authors: P J KELLY; W J MARTIN; A SCHIRGER; L A WEED
Journal: JAMA Date: 1960-09-24 Impact factor: 56.272

2. A selective medium for the isolation of brucellae from contaminated materials.

Authors: C D KUZDAS; E V MORSE
Journal: J Bacteriol Date: 1953-10 Impact factor: 3.490

3. Brucellosis of the spine.

Authors: M M Glasgow
Journal: Br J Surg Date: 1976-04 Impact factor: 6.939

4. Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up.

Authors: E Gazapo; J Gonzalez Lahoz; J L Subiza; M Baquero; J Gil; E G de la Concha
Journal: J Infect Dis Date: 1989-02 Impact factor: 5.226

5. The development of a new selective medium for the isolation of Brucella abortus from contaminated sources.

Authors: I D Farrell
Journal: Res Vet Sci Date: 1974-05 Impact factor: 2.534

6. Selective media for the isolation of Brucella ovis.

Authors: G M Brown; C R Ranger; D J Kelley
Journal: Cornell Vet Date: 1971-04

7. Brucella osteomyelitis of the distal part of the femur. A case report.

Authors: M A Keenan; G G Guttmann
Journal: J Bone Joint Surg Am Date: 1982-01 Impact factor: 5.284

Review 8. Brucellosis in the United States, 1960-1972. An abattoir-associated disease. Part I. Clinical features and therapy.

Authors: T M Buchanan; L C Faber; R A Feldman
Journal: Medicine (Baltimore) Date: 1974-11 Impact factor: 1.889

Buffered charcoal-yeast extract medium for the isolation of brucellae.

1. Brucellosis of the bones and joints. Experience with thirty-six patients.

2. A selective medium for the isolation of brucellae from contaminated materials.

3. Brucellosis of the spine.

4. Changes in IgM and IgG antibody concentrations in brucellosis over time: importance for diagnosis and follow-up.

5. The development of a new selective medium for the isolation of Brucella abortus from contaminated sources.

6. Selective media for the isolation of Brucella ovis.

7. Brucella osteomyelitis of the distal part of the femur. A case report.

Review 8. Brucellosis in the United States, 1960-1972. An abattoir-associated disease. Part I. Clinical features and therapy.

9. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings.

10. Osteoarticular complications of brucellosis: a study of 169 cases.

Review 1. Bioterrorism: implications for the clinical microbiologist.

2. Selective buffered charcoal-yeast extract medium for isolation of nocardiae from mixed cultures.

3. Clinical demonstration of isolation of Nocardia asteroides on buffered charcoal-yeast extract media.