Literature DB >> 21161348

A new polyethyleneglycol-derivatized hemoglobin derivative with decreased oxygen affinity and limited toxicity.

Oana Zolog1, Augustin Mot, Florina Deac, Alina Roman, Eva Fischer-Fodor, Radu Silaghi-Dumitrescu.   

Abstract

A new protocol is described for derivatization of hemoglobin with polyethyleneglycol (PEG) via reaction of the unmodified native hemoglobin with an activated amine-reacting polyethylene glycol derivative which, unlike protocols previously described, leads to formation of a peptide bond between hemoglobin and PEG. Dioxygen binding and peroxide reactivities of the derivatized hemoglobin are examined, and found to be within reasonable limits, with the particular observation that, unlike with a few other derivatization protocols, the dioxygen affinity is slightly lower than that of native Hb. In cell culture tests (human umbilical vein epithelial cells, HUVEC), the derivatization protocol induces no toxic effect. These results show promise towards applicability for production of hemoglobin-based blood substitutes.

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Year:  2011        PMID： 21161348     DOI： 10.1007/s10930-010-9298-5

Source DB:  PubMed          Journal:  Protein J        ISSN： 1572-3887            Impact factor:   2.371


  17 in total

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4.  A novel antioxidant role for hemoglobin. The comproportionation of ferrylhemoglobin with oxyhemoglobin.

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Review 5.  A review of blood substitutes: examining the history, clinical trial results, and ethics of hemoglobin-based oxygen carriers.

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Authors:  I Portöro; L Kocsis; P Hermán; D Caccia; M Perrella; L Ronda; S Bruno; S Bettati; C Micalella; A Mozzarelli; A Varga; M Vas; K C Lowe; A Eke
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Review 9.  Oxygen therapeutics: can we tame haemoglobin?

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  4 in total

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Authors:  Vlad Al Toma; Anca D Farcaș; Ioana Roman; Bogdan Sevastre; Denisa Hathazi; Florina Scurtu; Grigore Damian; Radu Silaghi-Dumitrescu
Journal:  PLoS One       Date:  2016-04-20       Impact factor: 3.240

3.  The Reaction of Oxy Hemoglobin with Nitrite: Mechanism, Antioxidant-Modulated Effect, and Implications for Blood Substitute Evaluation.

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4.  Glutaraldehyde-Polymerized Hemoglobin: In Search of Improved Performance as Oxygen Carrier in Hemorrhage Models.

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  4 in total

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