OBJECTIVE AND DESIGN: This report describes a focused immediate early gene response by human umbilical vein endothelial cells (HUVEC) to Echis carinatus snake venom. MATERIALS OR SUBJECTS: Primary cultures of HUVEC were used to assess acute inflammatory gene responses. TREATMENTS: Crude E. carinatus venom (2.5 µg/ml) was used to stimulate HUVEC. RESULTS: HUVEC stimulated for 3 h with E. carinatus venom showed a focused response to the venom, with significant increases in metallothionein (e.g., MT1H, MT2A, MT1X) and cytochrome P450 (e.g., CYP1A1, CYP1B1) gene expressions compared to non-stimulated controls. Several other genes involved in cell growth and matrix attachment were repressed [e.g., thrombospondin 1 (THBS1), connective tissue growth factor (CTGF)]. CONCLUSIONS: These data suggest that acute vascular injury induced by hemorrhagic snake venom initiates an anti-oxidant response primarily involving metallothioneins.
OBJECTIVE AND DESIGN: This report describes a focused immediate early gene response by human umbilical vein endothelial cells (HUVEC) to Echis carinatus snake venom. MATERIALS OR SUBJECTS: Primary cultures of HUVEC were used to assess acute inflammatory gene responses. TREATMENTS: Crude E. carinatus venom (2.5 µg/ml) was used to stimulate HUVEC. RESULTS: HUVEC stimulated for 3 h with E. carinatus venom showed a focused response to the venom, with significant increases in metallothionein (e.g., MT1H, MT2A, MT1X) and cytochrome P450 (e.g., CYP1A1, CYP1B1) gene expressions compared to non-stimulated controls. Several other genes involved in cell growth and matrix attachment were repressed [e.g., thrombospondin 1 (THBS1), connective tissue growth factor (CTGF)]. CONCLUSIONS: These data suggest that acute vascular injury induced by hemorrhagic snake venom initiates an anti-oxidant response primarily involving metallothioneins.
Authors: Tony Yuen; Elisa Wurmbach; Robert L Pfeffer; Barbara J Ebersole; Stuart C Sealfon Journal: Nucleic Acids Res Date: 2002-05-15 Impact factor: 16.971
Authors: B R Pitt; M Schwarz; E S Woo; E Yee; K Wasserloos; S Tran; W Weng; R J Mannix; S A Watkins; Y Y Tyurina; V A Tyurin; V E Kagan; J S Lazo Journal: Am J Physiol Date: 1997-10
Authors: Carolina Alves Nicolau; Alyson Prorock; Yongde Bao; Ana Gisele da Costa Neves-Ferreira; Richard Hemmi Valente; Jay William Fox Journal: Toxins (Basel) Date: 2018-02-06 Impact factor: 4.546