| Literature DB >> 21153789 |
Abstract
This chapter is an overview of basic principles of fluorescence microscopy, including a brief history on the invention of this type of microscopy. The chapter highlights important points related to properties of fluorochromes, resolution in fluorescence microscopy, phase contrast and fluorescence, fluorescence filters, construction of a fluorescence microscope, and tips on the correct use of this equipment.Entities:
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Year: 2011 PMID： 21153789 DOI： 10.1007/978-1-60761-950-5_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745