Literature DB >> 21153783

Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy: testes as a model.

Hélio Chiarini-Garcia1, Gleydes Gambogi Parreira, Fernanda R C L Almeida.   

Abstract

Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.

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Year:  2011        PMID： 21153783     DOI： 10.1007/978-1-60761-950-5_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Comparison between the techniques of inclusion in glycol methacrylate (GMA)-based plastic resin and paraffin for evaluation intestinal morphometry in horses.

Authors:  Cíntia Ferreira; Maristela Silveira Palhares; Ubiratan Pereira de Melo; Helio Chiarini-Garcia; Renata de Pino Albuquerque Maranhão; Valentim Arabicano Gheller; Fabíola Oliveira Paes Leme
Journal:  Rev Bras Med Vet       Date:  2022-04-12

2.  Postnatal development of skeletal muscle in pigs with intrauterine growth restriction: morphofunctional phenotype and molecular mechanisms.

Authors:  Fernando Felicioni; Andreia D Pereira; Andre L Caldeira-Brant; Thais G Santos; Thais M D Paula; Diogo Magnabosco; Fernando P Bortolozzo; Stephen Tsoi; Michael K Dyck; Walter Dixon; Patricia M Martinelli; Erika C Jorge; Helio Chiarini-Garcia; Fernanda R C L Almeida
Journal:  J Anat       Date:  2020-01-29       Impact factor: 2.610

3.  Glycol methacrylate embedding for the histochemical study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum.

Authors:  A J W Pinto; I F G de Amorim; L J Pinheiro; I M V M Madeira; C C Souza; H Chiarini-Garcia; M V Caliari; W L Tafuri
Journal:  Eur J Histochem       Date:  2015-10-26       Impact factor: 3.188
  3 in total

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