Literature DB >> 21153715

A flexible approach to studying post-transcriptional gene regulation in stably transfected mammalian cells.

Ralph C Nichols1, John Botson, Xiao Wei Wang, B JoNell Hamilton, Jane E Collins, Victoria Uribe, Seth A Brooks, Moe Zan, William F C Rigby.   

Abstract

The study of post-transcriptional regulation is constrained by the technical limitations associated with both transient and stable transfection of chimeric reporter plasmids examining the activity of 3'-UTR cis-acting elements. We report the adaptation of a commercially available system that enables consistent stable integration of chimeric reporter cDNA into a single genomic site in which transcription is induced by tetracycline. Using this system, we demonstrate the tight control afforded by this system and its suitability in mapping the regulatory function of defined cis-acting elements in the human TNF 3'-UTR, as well as the distinct effects of serum starvation on transiently transfected and stably integrated chimeric reporter genes.

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Year:  2011        PMID： 21153715      PMCID： PMC3786348          DOI： 10.1007/s12033-010-9360-8

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  31 in total

1.  Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) is required for lipopolysaccharide stimulation of tumor necrosis factor alpha (TNF-alpha) translation: glucocorticoids inhibit TNF-alpha translation by blocking JNK/SAPK.

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Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

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3.  Functional association between promoter structure and transcript alternative splicing.

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4.  Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU-rich elements: implications for joint and gut-associated immunopathologies.

Authors:  D Kontoyiannis; M Pasparakis; T T Pizarro; F Cominelli; G Kollias
Journal:  Immunity       Date:  1999-03       Impact factor: 31.745

5.  MAPKAP kinase 2 is essential for LPS-induced TNF-alpha biosynthesis.

Authors:  A Kotlyarov; A Neininger; C Schubert; R Eckert; C Birchmeier; H D Volk; M Gaestel
Journal:  Nat Cell Biol       Date:  1999-06       Impact factor: 28.824

6.  The p38 MAP kinase pathway signals for cytokine-induced mRNA stabilization via MAP kinase-activated protein kinase 2 and an AU-rich region-targeted mechanism.

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Journal:  EMBO J       Date:  1999-09-15       Impact factor: 11.598

Review 7.  The biology of cachectin/TNF--a primary mediator of the host response.

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Journal:  Annu Rev Immunol       Date:  1989       Impact factor: 28.527

8.  Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin.

Authors:  E Carballo; W S Lai; P J Blackshear
Journal:  Science       Date:  1998-08-14       Impact factor: 47.728

9.  TNF-alpha induction by LPS is regulated posttranscriptionally via a Tpl2/ERK-dependent pathway.

Authors:  C D Dumitru; J D Ceci; C Tsatsanis; D Kontoyiannis; K Stamatakis; J H Lin; C Patriotis; N A Jenkins; N G Copeland; G Kollias; P N Tsichlis
Journal:  Cell       Date:  2000-12-22       Impact factor: 41.582

10.  A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency.

Authors:  G A Taylor; E Carballo; D M Lee; W S Lai; M J Thompson; D D Patel; D I Schenkman; G S Gilkeson; H E Broxmeyer; B F Haynes; P J Blackshear
Journal:  Immunity       Date:  1996-05       Impact factor: 31.745
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  4 in total

1.  Peptides modeled on the RGG domain of AUF1/hnRNP-D regulate 3' UTR-dependent gene expression.

Authors:  Abigail Fellows; Bin Deng; Dale F Mierke; R Brooks Robey; Ralph C Nichols
Journal:  Int Immunopharmacol       Date:  2013-06-05       Impact factor: 4.932

2.  AUF1-RGG peptides up-regulate the VEGF antagonist, soluble VEGF receptor-1 (sFlt-1).

Authors:  Abigail Fellows; Dale F Mierke; Ralph C Nichols
Journal:  Cytokine       Date:  2013-06-14       Impact factor: 3.861

3.  AUF1/hnRNP D represses expression of VEGF in macrophages.

Authors:  Abigail Fellows; Mary E Griffin; Brenda L Petrella; Lihui Zhong; Fatemeh P Parvin-Nejad; Roy Fava; Peter Morganelli; R Brooks Robey; Ralph C Nichols
Journal:  Mol Biol Cell       Date:  2012-02-29       Impact factor: 4.138

4.  A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells.

Authors:  Sooncheol Lee; Douglas Micalizzi; Samuel S Truesdell; Syed I A Bukhari; Myriam Boukhali; Jennifer Lombardi-Story; Yasutaka Kato; Min-Kyung Choo; Ipsita Dey-Guha; Fei Ji; Benjamin T Nicholson; David T Myers; Dongjun Lee; Maria A Mazzola; Radhika Raheja; Adam Langenbucher; Nicholas J Haradhvala; Michael S Lawrence; Roopali Gandhi; Christopher Tiedje; Manuel D Diaz-Muñoz; David A Sweetser; Ruslan Sadreyev; David Sykes; Wilhelm Haas; Daniel A Haber; Shyamala Maheswaran; Shobha Vasudevan
Journal:  Genome Biol       Date:  2020-02-10       Impact factor: 13.583
  4 in total

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