FISHing chromosomes in endocrinology.

Fluorescencein situ hybridization (FISH) is currently recognized as a reliable, sensitive, and reproducible technique for identification of copy number and structure of chromosomes providing information on the single-cell level. The technique permits cytogenetic investigation of metaphase spreads and interphase nuclei. Several protocols have been used for preparations from fresh samples or archival material. Alphoid or telomeric DNA probes can detect specific chromosomes, and cosmid probes can localize single copies of the segment of interest. FISH is a powerful tool in understanding physiologic mechanisms, in generating high-resolution physical genetic maps, and in resolving problems of the pathogenesis of several diseases. FISH may shed light on the cytogenetic background and chromosomal alterations in the field of endocrinology, resulting in a better understanding of functional activities and various endocrine disorders.