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Literature DB >> 2115025

Isolation and characterization of an elastinolytic proteinase from Aspergillus flavus.

Abstract

An elastinolytic proteinase of Aspergillus flavus has been isolated to homogeneity, and its physical and biochemical properties have been characterized. Two purification protocols were compared; an initial step of ion-exchange chromatography was found to be equivalent to ammonium sulfate precipitation at neutral pH. A combination of gel filtration and adsorption chromatographies on the resultant crude enzyme produced highly purified elastase with yields of 5 to 10%. The enzyme is a 23-kilodalton protein with a pI of 7.6. The enzyme activity is markedly inhibited by numerous metal ions. Aspergillus elastase appears to be a metalloproteinase EC 3.4.24.X), as determined by its sensitivity to 1,10-phenanthroline.

Entities: Chemical Species

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Year: 1990 PMID： 2115025 PMCID： PMC258851 DOI： 10.1128/iai.58.8.2529-2534.1990

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

29 in total

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Journal: Infect Immun Date: 1979-05 Impact factor: 3.441

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Journal: Can J Biochem Date: 1965-10

15 in total

1. Zinc-regulated biosynthesis of immunodominant antigens from Aspergillus spp.

Authors: M Segurado; R López-Aragón; J A Calera; J M Fernández-Abalos; F Leal
Journal: Infect Immun Date: 1999-05 Impact factor: 3.441

2. Extracellular proteome of Aspergillus terreus grown on different carbon sources.

Authors: Mee-Jung Han; Nag-Jong Kim; Sang Yup Lee; Ho Nam Chang
Journal: Curr Genet Date: 2010-06-08 Impact factor: 3.886

Review 3. Proteomics as a Tool to Identify New Targets Against Aspergillus and Scedosporium in the Context of Cystic Fibrosis.

Authors: Andoni Ramirez-Garcia; Aize Pellon; Idoia Buldain; Aitziber Antoran; Aitana Arbizu-Delgado; Xabier Guruceaga; Aitor Rementeria; Fernando L Hernando
Journal: Mycopathologia Date: 2017-05-08 Impact factor: 2.574

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Authors: L H Hogan; B S Klein; S M Levitz
Journal: Clin Microbiol Rev Date: 1996-10 Impact factor: 26.132

5. Purification and characterization of an elastinolytic metalloprotease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung.

Authors: A Markaryan; I Morozova; H Yu; P E Kolattukudy
Journal: Infect Immun Date: 1994-06 Impact factor: 3.441

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Authors: M Frosco; T Chase; J D Macmillan
Journal: Infect Immun Date: 1992-03 Impact factor: 3.441

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Authors: M Monod; S Paris; D Sanglard; K Jaton-Ogay; J Bille; J P Latgé
Journal: Infect Immun Date: 1993-10 Impact factor: 3.441

8. Molecular cloning of the cDNA and gene for an elastinolytic aspartic proteinase from Aspergillus fumigatus and evidence of its secretion by the fungus during invasion of the host lung.

Authors: J D Lee; P E Kolattukudy
Journal: Infect Immun Date: 1995-10 Impact factor: 3.441

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Authors: M B Frosco; T Chase; J D Macmillan
Journal: Mycopathologia Date: 1994-02 Impact factor: 2.574

10. Molecular cloning and sequencing of the cDNA and gene for a novel elastinolytic metalloproteinase from Aspergillus fumigatus and its expression in Escherichia coli.

Authors: T D Sirakova; A Markaryan; P E Kolattukudy
Journal: Infect Immun Date: 1994-10 Impact factor: 3.441