Literature DB >> 2114822

Localization of glucoamylase genes of Saccharomyces cerevisiae by pulsed field gel electrophoresis.

G R Bignell1, I H Evans.   

Abstract

The chromosomal locations of four glucoamylase-specifying genes in the yeast Saccharomyces cerevisiae have been determined. Chromosomes were separated by pulsed field gel electrophoresis and blots were probed with radiolabelled STA2 and marker DNA from specific yeast chromosomes. The three genes encoding extracellular glucoamylases, STA1 (DEX2), STA2 (DEX1) and STA3 (DEX3) are located on chromosomes IV, II and XIV, respectively. SGA, specifying the sporulation-specific glucoamylase, was positioned on chromosome IX.

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Year:  1990        PMID: 2114822     DOI: 10.1007/bf02388079

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN: 0003-6072            Impact factor:   2.271


  14 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Glucoamylases from Saccharomyces diastaticus.

Authors:  J A Erratt
Journal:  Crit Rev Biotechnol       Date:  1987       Impact factor: 8.429

3.  Inheritance of chromosome length polymorphisms in Saccharomyces cerevisiae.

Authors:  B Ono; Y Ishino-Arao
Journal:  Curr Genet       Date:  1988-11       Impact factor: 3.886

4.  A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625.

Authors:  D Patel; I H Evans; E A Bevan
Journal:  Curr Genet       Date:  1990-04       Impact factor: 3.886

5.  A new mapping method employing a meiotic rec-mutant of yeast.

Authors:  S Klapholz; R E Esposito
Journal:  Genetics       Date:  1982-03       Impact factor: 4.562

6.  Gene fusion is a possible mechanism underlying the evolution of STA1.

Authors:  I Yamashita; M Nakamura; S Fukui
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

7.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

8.  A DEX gene conferring production of extracellular amyloglucosidase on yeast.

Authors:  P Meaden; K Ogden; H Bussey; R S Tubb
Journal:  Gene       Date:  1985       Impact factor: 3.688

9.  An electrophoretic karyotype for yeast.

Authors:  G F Carle; M V Olson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

10.  Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus.

Authors:  I Yamashita; K Suzuki; S Fukui
Journal:  J Bacteriol       Date:  1985-02       Impact factor: 3.490

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  2 in total

1.  Transfer of genes for utilization of starch (sta2) and melibiose (mel) to industrial strains of Saccharomyces cerevisiae by single-chromosome transfer, using a kar1 mutant as vector.

Authors:  J F Spencer; D M Spencer; L de Figueroa; J M Nougues; H Heluane
Journal:  Appl Microbiol Biotechnol       Date:  1992-05       Impact factor: 4.813

Review 2.  A re-evaluation of diastatic Saccharomyces cerevisiae strains and their role in brewing.

Authors:  Kristoffer Krogerus; Brian Gibson
Journal:  Appl Microbiol Biotechnol       Date:  2020-03-13       Impact factor: 4.813

  2 in total

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