OBJECTIVES: To evaluate in vitro antitumor effects of bee honey (BH) and Nigella sativa (NS) on HepG2 through their antioxidant and apoptotic activities. METHODS: HepG2 cell line was treated with different concentrations of diluted unfractionated BH and different concentrations of alcohol extract of NS. Exposure lasted for different time durations (6-72 hours), both dose-response and time course-response were conducted. Cell viability was tested by trypan blue exclusion test. Total antioxidant status and caspase-3 activity were estimated in the cell lysate. Nitric oxide levels were measured in culture supernatants of both treated and untreated HepG2 at all indicated times. RESULTS: Treatment of HepG2 cells with BH and NS leads to a significant decrease in both the number of viable HepG2 cells and the levels of nitric oxide on one hand, but improvement of the total antioxidant status and caspase-3 activity on the other, especially in HepG2 cells treated with higher doses of BH and NS (20% and 5000 μg/mL, respectively) and for longer duration (72 hours). CONCLUSIONS: BH and NS are effective in reducing the viability of HepG2 cells, improving their antioxidant status and inducing their apoptotic death.
OBJECTIVES: To evaluate in vitro antitumor effects of bee honey (BH) and Nigella sativa (NS) on HepG2 through their antioxidant and apoptotic activities. METHODS: HepG2 cell line was treated with different concentrations of diluted unfractionated BH and different concentrations of alcohol extract of NS. Exposure lasted for different time durations (6-72 hours), both dose-response and time course-response were conducted. Cell viability was tested by trypan blue exclusion test. Total antioxidant status and caspase-3 activity were estimated in the cell lysate. Nitric oxide levels were measured in culture supernatants of both treated and untreated HepG2 at all indicated times. RESULTS: Treatment of HepG2 cells with BH and NS leads to a significant decrease in both the number of viable HepG2 cells and the levels of nitric oxide on one hand, but improvement of the total antioxidant status and caspase-3 activity on the other, especially in HepG2 cells treated with higher doses of BH and NS (20% and 5000 μg/mL, respectively) and for longer duration (72 hours). CONCLUSIONS:BH and NS are effective in reducing the viability of HepG2 cells, improving their antioxidant status and inducing their apoptotic death.
Authors: Mohammad A I Al-Hatamleh; Walhan Alshaer; Ma'mon M Hatmal; Lidawani Lambuk; Naveed Ahmed; Mohd Zulkifli Mustafa; Siew Chun Low; Juhana Jaafar; Khalid Ferji; Jean-Luc Six; Vuk Uskoković; Rohimah Mohamud Journal: Front Mol Biosci Date: 2022-04-11
Authors: Yazan Ranneh; Abdah Md Akim; Hasiah Ab Hamid; Huzwah Khazaai; Abdulmannan Fadel; Zainul Amiruddin Zakaria; Mohammed Albujja; Mohd Fadzelly Abu Bakar Journal: BMC Complement Med Ther Date: 2021-01-14
Authors: Priyanka Aryappalli; Sarah S Al-Qubaisi; Samir Attoub; Junu A George; Kholoud Arafat; Khalil B Ramadi; Yassir A Mohamed; Mezoon M Al-Dhaheri; Ashraf Al-Sbiei; Maria J Fernandez-Cabezudo; Basel K Al-Ramadi Journal: Front Oncol Date: 2017-08-14 Impact factor: 6.244