| Literature DB >> 21147066 |
Shih-Ching Chan1, Shih-Yen Lo, Je-Wen Liou, Min-Ching Lin, Ciao-Ling Syu, Meng-Jiun Lai, Yi-Cheng Chen, Hui-Chun Li.
Abstract
Hepatitis C viral RNA synthesis has been demonstrated to occur on a lipid raft membrane structure. Lipid raft membrane fraction purified by membrane flotation analysis was observed using transmission electron microscopy and atomic force microscopy. Particles around 0.7 um in size were found in lipid raft membrane fraction purified from hepatitis C virus (HCV) replicon but not their parental HuH7 cells. HCV NS5A protein was associated with these specialized particles. After several cycles of freezing-thawing, these particles would fuse into larger sizes up to 10 um. Knockdown of seven proteins associated with lipid raft (VAPA, COPG, RAB18, COMT, CDC42, DPP4, and KDELR2) of HCV replicon cells reduced the observed number of these particles and suppressed the HCV replication. Results in this study indicated that HCV replication complexes with associated lipid raft membrane form distinct particle structures of around 0.7 um as observed from transmission electron microscopy and atomic force microscopy. Copyright ÂEntities:
Mesh：
Substances：
Year: 2010 PMID： 21147066 DOI： 10.1016/j.bbrc.2010.12.037
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575