Literature DB >> 21146491

Optimization of the scratch assay for in vitro skeletal muscle wound healing analysis.

K P Goetsch1, C U Niesler.   

Abstract

Myoblast migration is essential for muscle development, regeneration, and repair. Various assays are available for the study of migration; however, many are not suitable for the analysis of myoblast migration during skeletal muscle wound repair. Furthermore, versatility (e.g., to investigate the effect of the extracellular matrix on cell movement) is limited within these methods. Here we report the optimization of the scratch assay specifically for two-dimensional myoblast migration and discuss the advantages of this assay in comparison with other commercially available methods.
Copyright © 2010 Elsevier Inc. All rights reserved.

Mesh：

Year:  2010        PMID： 21146491     DOI： 10.1016/j.ab.2010.12.012

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  24 in total

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2.  Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract.

Authors:  Nicholas Cormier; Alexander Yeo; Elizabeth Fiorentino; Julia Paxson
Journal:  J Vis Exp       Date:  2015-12-03       Impact factor: 1.355

Review 3.  In vitro myoblast motility models: investigating migration dynamics for the study of skeletal muscle repair.

Authors:  K P Goetsch; K H Myburgh; Carola U Niesler
Journal:  J Muscle Res Cell Motil       Date:  2013-10-23       Impact factor: 2.698

4.  In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration.

Authors:  Bronson I Pinto; Nathan D Cruz; Oscar R Lujan; Catherine R Propper; Robert S Kellar
Journal:  J Vis Exp       Date:  2019-02-23       Impact factor: 1.355

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Journal:  J Biol Chem       Date:  2012-09-19       Impact factor: 5.157

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8.  Sensitivity of edge detection methods for quantifying cell migration assays.

Authors:  Katrina K Treloar; Matthew J Simpson
Journal:  PLoS One       Date:  2013-06-24       Impact factor: 3.240

9.  Thermosensitive Betulinic Acid-Loaded Magnetoliposomes: A Promising Antitumor Potential for Highly Aggressive Human Breast Adenocarcinoma Cells Under Hyperthermic Conditions.

Authors:  Claudia Geanina Farcas; Cristina Dehelean; Iulia Andreea Pinzaru; Marius Mioc; Vlad Socoliuc; Elena-Alina Moaca; Stefana Avram; Roxana Ghiulai; Dorina Coricovac; Ioana Pavel; Praveen Kumar Alla; Octavian Marius Cretu; Codruta Soica; Felicia Loghin
Journal:  Int J Nanomedicine       Date:  2020-10-23

10.  Enhanced keratinocyte proliferation and migration in co-culture with fibroblasts.

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Journal:  PLoS One       Date:  2012-07-20       Impact factor: 3.240
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