OBJECTIVE: The aim of this study was to assess by light microscopy changes in alveolar bone during orthodontic movement in rats. BACKGROUND: Orthodontic movement causes both removal and deposition of bone tissue. The use of laser phototherapy (LPT) is considered an enhancement factor for bone repair. METHODS: Thirty Wistar rats were divided into two groups (n = 15) and subdivided according to animal death (7,13, and 19 days). Half of the animals in each group were treated with LPT during orthodontic movement. After animal death, specimens were processed and underwent histological and semi-quantitative analyses (HE and Sirius red). RESULTS: LPT-irradiated specimens showed significantly higher numbers of osteoclasts when compared with controls at both 7 (p = 0.015) and 19 (p = 0.007) days, as well as significant increases in the number of osteoblasts (p = 0.015) between days 7 and 13. The amount of collagen matrix was significantly reduced between days 7 and 13 at both pressure and tension sites in controls (p = 0.015) but not in LPT-treated animals. LPT-treated subjects showed significantly greater deposition of collagen matrix at the pressure site at both the thirteenth (p = 0.007) and nineteenth days (p = 0.001). At the tension site, a significant increase in the amount of collagen matrix was observed in non-irradiated specimens (p = 0.048) between days 7 and 19. CONCLUSIONS: LPT caused significant histological changes in the alveolar bone during induced tooth movement, including alterations in the number of both osteoclasts and osteoblasts and in collagen deposition in both pressure and tension areas.
OBJECTIVE: The aim of this study was to assess by light microscopy changes in alveolar bone during orthodontic movement in rats. BACKGROUND: Orthodontic movement causes both removal and deposition of bone tissue. The use of laser phototherapy (LPT) is considered an enhancement factor for bone repair. METHODS: Thirty Wistar rats were divided into two groups (n = 15) and subdivided according to animal death (7,13, and 19 days). Half of the animals in each group were treated with LPT during orthodontic movement. After animal death, specimens were processed and underwent histological and semi-quantitative analyses (HE and Sirius red). RESULTS: LPT-irradiated specimens showed significantly higher numbers of osteoclasts when compared with controls at both 7 (p = 0.015) and 19 (p = 0.007) days, as well as significant increases in the number of osteoblasts (p = 0.015) between days 7 and 13. The amount of collagen matrix was significantly reduced between days 7 and 13 at both pressure and tension sites in controls (p = 0.015) but not in LPT-treated animals. LPT-treated subjects showed significantly greater deposition of collagen matrix at the pressure site at both the thirteenth (p = 0.007) and nineteenth days (p = 0.001). At the tension site, a significant increase in the amount of collagen matrix was observed in non-irradiated specimens (p = 0.048) between days 7 and 19. CONCLUSIONS: LPT caused significant histological changes in the alveolar bone during induced tooth movement, including alterations in the number of both osteoclasts and osteoblasts and in collagen deposition in both pressure and tension areas.
Authors: Cristiane Becher Rosa; Fernando Antonio Lima Habib; Telma Martins de Araújo; Jean Nunes Dos Santos; Maria Cristina T Cangussu; Artur Felipe Santos Barbosa; Isabele Cardoso Vieira de Castro; Antônio Luiz Barbosa Pinheiro Journal: Lasers Med Sci Date: 2016-11-24 Impact factor: 3.161
Authors: Cristiane Becher Rosa; Fernando Antonio Lima Habib; Telma Martins de Araújo; Juliana Silveira Aragão; Rafael Soares Gomes; Artur Felipe Santos Barbosa; Landulfo Silveira; Antonio L B Pinheiro Journal: Lasers Med Sci Date: 2013-02-21 Impact factor: 3.161
Authors: Dayla Thyeme Higashi; Avacir Casanova Andrello; Pedro Marcelo Tondelli; Dari de Oliveira Toginho Filho; Solange de Paula Ramos Journal: Lasers Med Sci Date: 2016-10-29 Impact factor: 3.161
Authors: I Z Figueira; A P C Sousa; A W Machado; F A L Habib; L G P Soares; A L B Pinheiro Journal: Lasers Med Sci Date: 2018-08-18 Impact factor: 3.161