Wensong Zuo1, Juanhu Xu, Changxin Zhou, Lishe Gan. 1. Institute of Modern Chinese Medicine, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Abstract
OBJECTIVE: To established a novel HPLC-PAD method for the simultaneous determination of five compounds (camellianin A, camellianin B, apigenin, quercitrin, epicatechin) in leaves of Adinandra nitida. METHOD: These compounds were effectively separated on a reversed-phase C18 column (4.6 mm x 250 mm, 10 microm) with the column temperature at 25 degrees C. The mobile phase was composed of 0.1% aqueous formic acid and methanol. The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 280 nm before 15 min for detecting epicatechin and at 262 nm from 15 to 40 min for detecting the rest four compounds. RESULT: All the linearities were good (r > 0.9991) within their test ranges. The established method showed good precision with overall intra-day and interday RSD values less than 3.33% and 3.2%, respectively. The average recoveries were in the range of 96.08% to 100.30% with RSD from 2.0% to 4.0%. The limits of detection (LOD) and quantification (LOQ) in the range of ranged from 0.9 to 5.6 ng and 3.0 to 19.0 ng, respectively. CONCLUSION: This established method can be applied to evaluate the intrinsic quality of the leaves of A. nitida.
OBJECTIVE: To established a novel HPLC-PAD method for the simultaneous determination of five compounds (camellianin A, camellianin B, apigenin, quercitrin, epicatechin) in leaves of Adinandra nitida. METHOD: These compounds were effectively separated on a reversed-phase C18 column (4.6 mm x 250 mm, 10 microm) with the column temperature at 25 degrees C. The mobile phase was composed of 0.1% aqueous formic acid and methanol. The flow rate was 1.0 mL x min(-1), the detection wavelength was set at 280 nm before 15 min for detecting epicatechin and at 262 nm from 15 to 40 min for detecting the rest four compounds. RESULT: All the linearities were good (r > 0.9991) within their test ranges. The established method showed good precision with overall intra-day and interday RSD values less than 3.33% and 3.2%, respectively. The average recoveries were in the range of 96.08% to 100.30% with RSD from 2.0% to 4.0%. The limits of detection (LOD) and quantification (LOQ) in the range of ranged from 0.9 to 5.6 ng and 3.0 to 19.0 ng, respectively. CONCLUSION: This established method can be applied to evaluate the intrinsic quality of the leaves of A. nitida.